371732 | Anti-Gsα-Subunit, C-Terminal (385-394) Rabbit pAb

371732
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ma Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      371732-50UL
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          Glass bottle 50 ul
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          Description
          OverviewRecognizes both large and small forms of Gsα-subunit. Does not cross-react with Giα-1-, Giα-2-, Giα-3-, or Goα-subunits.
          Catalogue Number371732
          Brand Family Calbiochem®
          References
          ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
          Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
          Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
          Product Information
          FormLiquid
          FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
          Positive controlGsα-Subunit, His•Tag®, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)
          PreservativeNone
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000)
          Application CommentsDoes not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mammals
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          371732

          References

          Reference overview
          Kumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
          Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
          Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.

          Citations

          Title
        • Alexandre Charbonneau, et al. (2005) Alterations in hepatic glucagon receptor density and in Gsa and Gia2 protein content with diet-induced hepatic steatosis: effects of acute exercise. American Journal of Physiology Endocrinology and Metabolism 289, E8-E14.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-August-2007 RFH
          ApplicationImmunoblotting (1:1000)
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~40-45 kDa Gsα subunit protein.
          HostRabbit
          Immunogena synthetic peptide (RMHLRQYELL) (Cat. No. 371782) corresponding to amino acids at the C-terminus of mammalian Gsα subunit, conjugated to KLH
          IsotypeIgG
          Speciesmammalian
          Positive controlGsα-Subunit, His•Tag®, Rat Brain, Recombinant, E. coli (Cat. No. 371765) or Gsα-Subunit, Recombinant, E. coli, Immunoblot Standard (Cat. No. 371764)
          FormLiquid
          FormulationIn 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
          PreservativeNone
          CommentsDoes not cross-react with Giα-1, Giα-2, Giα-3, or Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesKumar, R., et al. 1994. J. Mol. Cell. Cardiol. 26, 1537.
          Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
          Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
          Citation
        • Alexandre Charbonneau, et al. (2005) Alterations in hepatic glucagon receptor density and in Gsa and Gia2 protein content with diet-induced hepatic steatosis: effects of acute exercise. American Journal of Physiology Endocrinology and Metabolism 289, E8-E14.