345860 Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10)

345860
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, H, M, R R Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      345860-100UG
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      Stocked 
      Discontinued
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.
          Catalogue Number345860
          Brand Family Calbiochem®
          SynonymsAnti-GFAP
          Application Data
          Detection of human glial fibrillary acidic protein by immunohistochemistry. Sample: Astrocytoma tissue. Primary antibody: Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) (Cat. No. 345860) (10 µg/ml). Detection: fluorescence.
          References
          ReferencesTohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
          Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
          Product Information
          FormLiquid
          FormulationIn PBS.
          Positive controlHuman or rat brain tissues
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoprecipitation
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesELISA (0.1-0.5 µg/ml)
          Immunoblotting (0.1-0.5 µg/ml)
          Frozen Sections (10-50 µg/ml)
          Immunoprecipitation (2-5 µg/ml)
          Paraffin Sections (10-50 µg/ml, see comments)
          Application CommentsProduct is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin's-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenbovine GFAP
          ImmunogenBovine Brain
          Clone2.2B10
          HostRat
          IsotypeIgG2a
          Species Reactivity
          • Bovine
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          345860

          References

          Reference overview
          Tohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
          Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-August-2007 RFH
          SynonymsAnti-GFAP
          ApplicationELISA (0.1-0.5 µg/ml)
          Immunoblotting (0.1-0.5 µg/ml)
          Frozen Sections (10-50 µg/ml)
          Immunoprecipitation (2-5 µg/ml)
          Paraffin Sections (10-50 µg/ml, see comments)
          Application Data
          Detection of human glial fibrillary acidic protein by immunohistochemistry. Sample: Astrocytoma tissue. Primary antibody: Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) (Cat. No. 345860) (10 µg/ml). Detection: fluorescence.
          DescriptionPurified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).
          HostRat
          Immunogen speciesBovine Brain
          Immunogenbovine GFAP
          Clone2.2B10
          IsotypeIgG2a
          Speciesbovine, human, rodent
          Positive controlHuman or rat brain tissues
          FormLiquid
          FormulationIn PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsProduct is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin's-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesTohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
          Lee, V.M., et al. 1984. J. Neurochem. 42, 25.