371726 | Anti-Giα-3- and Goα-Subunits, C-Terminal (345-354) Rabbit pAb

371726
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ma Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      371726-50UL
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
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      Available
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          Glass bottle 50 ul
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          Description
          OverviewRecognizes both Giα-3- and Goα-subunits. Does not cross-react with other G protein subunits.
          Catalogue Number371726
          Brand Family Calbiochem®
          References
          ReferencesRaymond, J.R., et al. 1993. Biochemistry 32, 11064.
          Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
          Product Information
          FormLiquid
          FormulationSerum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000)
          Application CommentsThe Giα-3- and Goα-subunits share the last four amino acids (CGLY) of the immunizing peptide. Does not cross-react with other G-proteins. The specificity and cross-reactivity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic peptide (KNNLKECGLY) corresponding to amino acids 345-354 of mammalian Giα-3, conjugated to KLH
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mammals
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          371726

          References

          Reference overview
          Raymond, J.R., et al. 1993. Biochemistry 32, 11064.
          Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-November-2017 JSW
          ApplicationImmunoblotting (1:1000)
          DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes Giα-3 and Goα subunit proteins.
          HostRabbit
          Immunogena synthetic peptide (KNNLKECGLY) corresponding to amino acids 345-354 of mammalian Giα-3, conjugated to KLH
          IsotypeIgG
          Speciesmammalian
          FormLiquid
          FormulationSerum diluted in 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
          CommentsThe Giα-3- and Goα-subunits share the last four amino acids (CGLY) of the immunizing peptide. Does not cross-react with other G-proteins. The specificity and cross-reactivity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesRaymond, J.R., et al. 1993. Biochemistry 32, 11064.
          Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol. 195, 215.