AP1165 Anti-FIS1 Mouse mAb (1G9)

AP1165
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      AP1165-50UG
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      Stocked 
      Discontinued
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      Available
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          50 μg
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          Description
          OverviewRecognizes the ~15 kDa FIS1 protein in A431 cells and human liver and small intestine tissue.
          Catalogue NumberAP1165
          Brand Family Calbiochem®
          SynonymsAnti-TTC11, Anti-Fission 1 Homolog
          Application Data
          Detection of human FIS1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (1-5 µg/ml). Detection: chemiluninescence.

          Detection of human FIS1 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (1-5 µg/ml). Detection: chemiluninescence.

          Detection of human FIS1 by immunoblotting. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (3 µg/ml). Detection: colorimetric (DAB).
          References
          Product Information
          FormLiquid
          FormulationIn PBS, pH 7.2.
          Positive controlHuman liver tissue, A-431 cells, Human small intestine tissue
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Paraffin Sections
          Application NotesELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenFull-length, recombinant human FIS1 (aa 1-153), expressed as a GST fusion protein
          Clone1G9
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Regulatory Review
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-FIS1 Mouse mAb (1G9) SDS

          Title

          Safety Data Sheet (SDS) 

          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-October-2010 RFH
          SynonymsAnti-TTC11, Anti-Fission 1 Homolog
          ApplicationELISA (see comments)
          Immunoblotting (1-5 µg/ml)
          Paraffin Sections (3 µg/ml)
          Application Data
          Detection of human FIS1 by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (1-5 µg/ml). Detection: chemiluninescence.

          Detection of human FIS1 by immunoblotting. Sample: Whole cell lysate from A-431 cells. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (1-5 µg/ml). Detection: chemiluninescence.

          Detection of human FIS1 by immunoblotting. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-FIS1 Mouse mAb (1G9) (Cat. No. AP1165) (3 µg/ml). Detection: colorimetric (DAB).
          DescriptionPurified mouse monoclonal antibody. Recognizes the ~15 kDa FIS1 protein.
          BackgroundTTC11 promotes the fragmentation of the mitochondrial network and its perinuclear clustering. It can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. It interacts with DNM1L. The C terminus is required for mitochondrial localization, while the N teminus is necessary for mitochondrial fission.
          HostMouse
          ImmunogenFull-length, recombinant human FIS1 (aa 1-153), expressed as a GST fusion protein
          Clone1G9
          IsotypeIgG2b
          Specieshuman
          Positive controlHuman liver tissue, A-431 cells, Human small intestine tissue
          FormLiquid
          FormulationIn PBS, pH 7.2.
          PreservativeNone
          CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Regulatory Review