GR01L Anti-EGFR (Ab-1) Mouse mAb (528)

GR01L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      GR01L-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the EGF receptor protein in A431 cells and normal skin.
          Catalogue NumberGR01L
          Brand Family Calbiochem®
          SynonymsAnti-Epidermal Growth Factor Receptor
          References
          ReferencesHunter T. 1984. Nature 311, 414.
          Masui, H., et al. 1984. Cancer Res. 44, 1002.
          Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
          Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
          Product Information
          FormLyophilized
          FormulationLyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
          Positive controlA-431 cells or skin tissue
          PreservativeNone
          Applications
          Application ReferencesOriginal Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.
          Key Applications Immunoprecipitation
          Neutralization Studies
          Paraffin Sections
          Flow Cytometry
          Frozen Sections
          Immunofluorescence
          Application NotesFlow Cytometry (5 µg/ml)
          Frozen Sections (2.5 µg/ml)
          Immunofluorescence (5 µg/ml)
          Immunoprecipitation (1 µg/sample)
          Neutralization Studies (see comments and application references)
          Paraffin Sections (see application references)
          Application CommentsThis antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpartially purified, human EGFR from human A431 cells
          ImmunogenHuman
          Clone528
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-EGFR (Ab-1) Mouse mAb (528) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-EGFR (Ab-1) Mouse mAb (528) Certificates of Analysis

          TitleLot Number
          GR01L

          References

          Reference overview
          Hunter T. 1984. Nature 311, 414.
          Masui, H., et al. 1984. Cancer Res. 44, 1002.
          Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
          Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-October-2007 JSW
          SynonymsAnti-Epidermal Growth Factor Receptor
          ApplicationFlow Cytometry (5 µg/ml)
          Frozen Sections (2.5 µg/ml)
          Immunofluorescence (5 µg/ml)
          Immunoprecipitation (1 µg/sample)
          Neutralization Studies (see comments and application references)
          Paraffin Sections (see application references)
          DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~170 kDa EGFR protein.
          BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
          HostMouse
          Immunogen speciesHuman
          Immunogenpartially purified, human EGFR from human A431 cells
          Clone528
          IsotypeIgG2a
          Specieshuman, not mouse, not rat
          Positive controlA-431 cells or skin tissue
          FormLyophilized
          FormulationLyophilized from 20 mM ammonium bicarbonate solution, 0.1 mg FAF-BSA.
          PreservativeNone
          CommentsThis antibody binds to A431, HeLa, and other human cells but not to rodent cells. This antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity. Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsWe recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage.
          Toxicity Standard Handling
          ReferencesHunter T. 1984. Nature 311, 414.
          Masui, H., et al. 1984. Cancer Res. 44, 1002.
          Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
          Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
          Application referencesOriginal Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies