AB2253 Anti-Doublecortin Antibody

AB2253
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M WB, IHC, ICC Gp Serum Polyclonal Antibody
      Description
      Catalogue NumberAB2253
      ReplacesAB5910
      DescriptionAnti-Doublecortin Antibody
      Alternate Names
      • Doublin
      • Lissencephalin-X
      • Lis-X
      Background InformationNeuronal migration protein doublecortin (UniProt: O43602; also known as Doublin, Lissencephalin-X, Lis-X) is encoded by the DCX (also known as DBCN, LISX) gene (Gene ID: 1641) in murine species. Doublecortin is a microtubule-associated protein that is expressed almost exclusively in immature neurons. Hence, it has emerged as an important marker of neurogenesis. It is highly expressed in newly produced cells in the neurogenic zones; the sub ventricular zone along the lateral ventricle and the subgranular zone (SGZ) of the dentate gyrus. Doublecortin binds to microtubules in migrating cells and as a result promote the movement of these cells. It is reported to direct neuronal migration by regulating the organization and stability of microtubules. Mutations in DCX gene are reported to cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia. Doublecortin may also be involved in cancer metastasis where unstable interactions between doublecortin and microtubules destabilize cytoskeletal organization leading to disorganized movements of cells that can lead to the uncontrolled migration of cancer cells. (Ref.: Ayanlaja, A.A., et al. (2017). Front. Mol. Neurosci. doi: 10.3389/fnmol.2017.00199)..
      References
      Product Information
      FormatSerum
      Control
      • Rat Brain lysate.
      PresentationGST-Depleted Sera with 0.05% NaN3 .
      Applications
      ApplicationAnti-Doublecortin Antibody, Cat. No. AB2253, is a highly specific guinea pig polyclonal antibody that targets Doublecortin and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Doublecortin in mouse brain and rat brain tissue sections.

      Immunocytochemistry:
      Tested on mouse primary neurons. Optimal dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant Peptide.
      EpitopeC-Terminus
      HostGuinea Pig
      SpecificityThis Guinea pig polyclonal antibody detects doublecortin in murine and rat species. It targets an epitope with in 17 amino acids from the C-terminal region.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse and Rat. Expected to react with human based on sequence homology. Reactivity with other species has not been determined.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryIn the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • DBCN
      • DC
      • Doublin
      • LISX
      • Lis-X
      • Lissencephalin-X
      • OTTHUMP00000062892
      • SCLH
      • XLIS
      • doublecortex
      • doublecortin
      • doublin
      • lissencephalin-X
      Purification MethodGST Depletion
      UniProt Number
      UniProt SummaryFUNCTION: Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.

      SUBUNIT STRUCTURE: Interacts with tubulin.

      SUBCELLULAR LOCATION: Cytoplasm.

      TISSUE SPECIFICITY: Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

      DISEASE: Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'. Ref.1 Ref.2 Ref.9 Ref.10 Ref.18 Ref.21

      Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal. Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19

      A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).

      SEQUENCE SIMILARITY: Contains 2 doublecortin domains.

      Molecular Weight~45 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Doublecortin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Doublecortin Antibody Certificates of Analysis

      TitleLot Number
      Anti-Doublecortin2477980
      Anti-Doublecortin2455684
      Anti-Doublecortin - 23989612398961
      Anti-Doublecortin - 24287192428719
      Anti-Doublecortin - 20204582020458
      Anti-Doublecortin - 20736432073643
      Anti-Doublecortin - 21961112196111
      Anti-Doublecortin - 22668052266805
      Anti-Doublecortin - 23411192341119
      Anti-Doublecortin - 25177972517797

      References

      Reference overviewPub Med ID
      Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
      Edri, R; Yaffe, Y; Ziller, MJ; Mutukula, N; Volkman, R; David, E; Jacob-Hirsch, J; Malcov, H; Levy, C; Rechavi, G; Gat-Viks, I; Meissner, A; Elkabetz, Y
      Nature communications  6  6500  2015

      Show Abstract
      25799239 25799239
      Aberrant hippocampal neurogenesis contributes to epilepsy and associated cognitive decline.
      Cho, KO; Lybrand, ZR; Ito, N; Brulet, R; Tafacory, F; Zhang, L; Good, L; Ure, K; Kernie, SG; Birnbaum, SG; Scharfman, HE; Eisch, AJ; Hsieh, J
      Nature communications  6  6606  2015

      Show Abstract
      25808087 25808087
      Combination treatment with ethyl pyruvate and IGF-I exerts neuroprotective effects against brain injury in a rat model of neonatal hypoxic-ischemic encephalopathy.
      Rong, Z; Pan, R; Chang, L; Lee, W
      International journal of molecular medicine  36  195-203  2015

      Show Abstract
      25999282 25999282
      Systemic attenuation of the TGF-β pathway by a single drug simultaneously rejuvenates hippocampal neurogenesis and myogenesis in the same old mammal.
      Yousef, H; Conboy, MJ; Morgenthaler, A; Schlesinger, C; Bugaj, L; Paliwal, P; Greer, C; Conboy, IM; Schaffer, D
      Oncotarget  6  11959-78  2015

      Show Abstract
      26003168 26003168
      Identification of distinct ChAT⁺ neurons and activity-dependent control of postnatal SVZ neurogenesis.
      Paez-Gonzalez, P; Asrican, B; Rodriguez, E; Kuo, CT
      Nature neuroscience  17  934-42  2014

      Show Abstract
      24880216 24880216
      Analysis of Mll1 deficiency identifies neurogenic transcriptional modules and Brn4 as a factor for direct astrocyte-to-neuron reprogramming.
      Potts, MB; Siu, JJ; Price, JD; Salinas, RD; Cho, MJ; Ramos, AD; Hahn, J; Margeta, M; Oldham, MC; Lim, DA
      Neurosurgery  75  472-82; discussion 482  2014

      Show Abstract
      24887289 24887289
      EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons.
      Puehringer, D; Orel, N; Lüningschrör, P; Subramanian, N; Herrmann, T; Chao, MV; Sendtner, M
      Nature neuroscience  16  407-15  2013

      Show Abstract
      23416450 23416450
      TGF-β superfamily gene expression and induction of the Runx1 transcription factor in adult neurogenic regions after brain injury.
      Logan, TT; Villapol, S; Symes, AJ
      PloS one  8  e59250  2013

      Show Abstract
      23555640 23555640
      Calorie restriction alleviates the age-related decrease in neural progenitor cell division in the aging brain.
      Park, JH; Glass, Z; Sayed, K; Michurina, TV; Lazutkin, A; Mineyeva, O; Velmeshev, D; Ward, WF; Richardson, A; Enikolopov, G
      The European journal of neuroscience  37  1987-93  2013

      Show Abstract
      23773068 23773068
      Onecut1 is essential for horizontal cell genesis and retinal integrity.
      Wu, F; Li, R; Umino, Y; Kaczynski, TJ; Sapkota, D; Li, S; Xiang, M; Fliesler, SJ; Sherry, DM; Gannon, M; Solessio, E; Mu, X
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  13053-65, 13065a  2013

      Show Abstract
      23926259 23926259