MAB3299 Anti-DNA Antibody, single stranded specific, clone F7-26

MAB3299
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A FC, ICC, IHC, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB3299
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-DNA Antibody, single stranded specific, clone F7-26
      Background InformationApoptosis is a genetically and biochemically regulated mechanism of programmed cell death that plays an important role in a variety of human diseases including cancer, immune disorders, and neurologic, cardiovascular and infectious diseases. Decreased stability of apoptotic DNA toward thermal denaturation is induced by the proteolysis of DNA-bound proteins during the execution of apoptosis. This conclusion is supported by the binding of anti-ssDNA monoclonal antibodies to non-apoptotic cells treated with proteinase K before heating and by prevention of DNA denaturation and MAb reactivity in apoptotic nuclei reconstituted with histones (Frankfurt et al. 1996, 1997). Thus, staining of cell suspensions and tissue sections with such antibodies following a heat treatment, which induces DNA denaturation in situ only in apoptotic nuclei, is a specific and sensitive method for the detection of apoptotic cells. CHEMICON MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types.
      References
      Product Information
      FormatPurified
      Control
      • Apoptotic cells
      PresentationPurified Immunoglobulin. Liquid in PBS supplemented with 5% fetal bovine Serum (FBS), containing no preservatives.
      Applications
      ApplicationAnti-DNA Antibody, single stranded specific, clone F7-26 detects level of DNA & has been published & validated for use in FC, IC, IH, IH(P).
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application Notes1. Immunoprecipitation

      2. Immunohistochemistry: formalin fixed, paraffin embedded tissues & 4% PF frozen tissues (special detection protocol required. FACS analysis (special protocol required),
      Biological Information
      ImmunogenF7-26 was generated by the immunization of mice with calf thymus single-stranded DNA.
      Epitopesingle stranded specific
      CloneF7-26
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySpecifically reactive with single-stranded DNA and is ideal for the detection of apoptotic cells in suspension or in tissue sections. The antibody does not recognize DNA in double-stranded conformations. MAB3299 provides a cellular marker specific for apoptotic death that is independent of internucleosomal DNA fragmentation and is useful for the detection of different stages of apoptosis in various cell types. Antibody reacts specifically with deoxycytidine and requires stretch of ssDNA of at least 25-30 bases in length for the binding. Importantly, in contrast with the TUNEL method, monoclonal antibodies to ssDNA are specific for apoptotic cell death and do not detect necrotic cells.
      IsotypeIgM
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodAmmonium Sulfate Precipitation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -80°C in undiluted aliquots for up to 12 months after date of purchase.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-DNA Antibody, single stranded specific, clone F7-26 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-DNA Antibody, single stranded specific, clone F7-26 Certificates of Analysis

      TitleLot Number
      Anti-DNA, single stranded specific, -27950882795088
      Anti-DNA, single stranded specific, clone F7-262465151
      Anti-DNA, single stranded specific, clone F7-26 - 24264792426479
      Anti-DNA, single stranded specific, clone F7-26 - 19908881990888
      Anti-DNA, single stranded specific, clone F7-26 - 20111252011125
      Anti-DNA, single stranded specific, clone F7-26 - 20901122090112
      Anti-DNA, single stranded specific, clone F7-26 - 22938642293864
      Anti-DNA, single stranded specific, clone F7-26 - 23177592317759
      Anti-DNA, single stranded specific, clone F7-26 - 23325022332502
      Anti-DNA, single stranded specific, clone F7-26 - 30506193050619

      References

      Reference overviewPub Med ID
      Suppression of tumor growth in mice by rationally designed pseudopeptide inhibitors of fibroblast activation protein and prolyl oligopeptidase.
      Jackson, KW; Christiansen, VJ; Yadav, VR; Silasi-Mansat, R; Lupu, F; Awasthi, V; Zhang, RR; McKee, PA
      Neoplasia (New York, N.Y.)  17  43-54  2015

      Show Abstract
      25622898 25622898
      Chromatin collapse during caspase-dependent apoptotic cell death requires DNA fragmentation factor, 40-kDa subunit-/caspase-activated deoxyribonuclease-mediated 3'-OH single-strand DNA breaks.
      Iglesias-Guimarais, V; Gil-Guiñon, E; Sánchez-Osuna, M; Casanelles, E; García-Belinchón, M; Comella, JX; Yuste, VJ
      The Journal of biological chemistry  288  9200-15  2013

      Show Abstract
      23430749 23430749
      Involvement of autophagy in the pharmacological effects of the mTOR inhibitor everolimus in acute kidney injury.
      Shunsaku Nakagawa,Kumiko Nishihara,Ken-Ichi Inui,Satohiro Masuda
      European journal of pharmacology  696  2012

      Show Abstract
      23022334 23022334
      Apnea produces excitotoxic hippocampal synapses and neuronal apoptosis.
      Simon J Fung,Mingchu Xi,Jianhua Zhang,Sharon Sampogna,Michael H Chase
      Experimental neurology  238  2012

      Show Abstract
      22921462 22921462
      Heat shock protein 27 confers resistance to androgen ablation and chemotherapy in prostate cancer cells through eIF4E.
      C Andrieu,D Taieb,V Baylot,S Ettinger,P Soubeyran,A De-Thonel,C Nelson,C Garrido,A So,L Fazli,F Bladou,M Gleave,J L Iovanna,P Rocchi
      Oncogene  29  2010

      Show Abstract
      20101233 20101233
      Method of specific detection of apoptosis using formamide-induced DNA denaturation assay.
      Ito, Y; Shibata, MA; Kusakabe, K; Otsuki, Y
      The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society  54  683-92  2006

      Show Abstract
      16714424 16714424
      Small interference RNA targeting heat-shock protein 27 inhibits the growth of prostatic cell lines and induces apoptosis via caspase-3 activation in vitro.
      Palma Rocchi, Paul Jugpal, Alan So, Shannon Sinneman, Susan Ettinger, Ladan Fazli, Colleen Nelson, Martin Gleave
      BJU international  98  1082-9  2006

      Show Abstract
      16879439 16879439
      Increased airway epithelial and T-cell apoptosis in COPD remains despite smoking cessation.
      Hodge, S; Hodge, G; Holmes, M; Reynolds, PN
      The European respiratory journal  25  447-54  2005

      Show Abstract
      15738287 15738287
      Increased peripheral blood T-cell apoptosis and decreased Bcl-2 in chronic obstructive pulmonary disease.
      Hodge, S; Hodge, G; Holmes, M; Reynolds, PN
      Immunology and cell biology  83  160-6  2005

      Show Abstract
      15748212 15748212
      A novel Mtd splice isoform is responsible for trophoblast cell death in pre-eclampsia.
      Soleymanlou, N; Wu, Y; Wang, JX; Todros, T; Ietta, F; Jurisicova, A; Post, M; Caniggia, I
      Cell death and differentiation  12  441-52  2005

      Show Abstract
      15775999 15775999