69050 Anti-Cre Antibody

69050
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      69050-3
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          Plastic ampoule 50 ul
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          Description
          OverviewRecombinase cre (UniProt: P06956; also known as Cre) is encoded by the cre gene (Gene ID: 2777477) in Escherichia phage P1 (Bacteriophage P1). Cre (Causes recombination with Cyclization recombinase), a 38 kDa DNA recombinase of the 'phage' integrase family, is derived from the P1 bacteriophage. It catalyzes site-specific recombination and is highly specific for a 34-bp DNA sequence motif called loxP (locus of X-over P1) found in P1 DNA. The loxP site is composed of a variable asymmetric 8-bp sequence in between two sets of 13-bp palindromic sequences (ATAACTTCGTATA-NNNTANNN-TATACGAAGTTAT). Two cre recombinase proteins, each bind to one of the two 13-bp palindromic sequences at a loxP site, form a dimer. This dimer then binds to a second dimer on another loxP site to form a tetramer. The two sites joined by the tetramer are parallel in orientation and the double stranded DNA is cut at both loxP sites. The strands are then rejoined with DNA ligase. Because the Cre gene and loxP sites are not native to most species, they are often introduced into target cells or tissues and employed as a means to precisely control the expression of genes both in cultured cells in vitro and in animals in vivo.
          Supplied as purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide and 50% glycerol.

          Evaluated by Western Blotting with Recombinant Cre-His.

          Western Blotting Analysis: Representative lot data. Recombinant Cre-His loaded at 20 ng/lane (Lane 1) and 10 ng/lane (Lane 2) was probed with Cat. No. 69050-3, Anti-Cre (1:10,000 dilution). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Cre (~39 kDa).
          Catalogue Number69050
          Brand Family Novagen®
          References
          Product Information
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          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
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          Anti-Cre Antibody SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Cre Antibody Certificates of Analysis

          TitleLot Number
          69050

          Citations

          Title
        • Qian, J.Y., et al. 2008. Hypertension 51, 560.
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        • Xie, y., et al. 2006. Journal of Biological Chemistry 281, 4075.
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        • Izumi, T., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 5739.
        • Koike, C., et al. 2005. Journal of Neuroscience 25, 10290.
        • Moeller, M.J., et al. 2005. American Journal of Physiology: Renal 289, F481.
        • Pfister, O., et al. 2005. Circulation Research 97, 52.
        • Schulein, R., et al. 2005. Procedings of the National Academy of Science 102, 856.
        • Peitz, M., et al. 2002. Procedings of the National Academy of Science 99, 4489.
        • Takebayashi, H., et al. 2002. Current Biology 12, 1157.
        • Morimura, T., et al. 2000. Journal of Biological Chemistry 275, 36523.
        • Selbert, S., et al. 1998. Transgenic Research 7, 387.
        • Giovannini, et. al. 2000. Genes and Development 14, 1617.
        • User Protocols

          Title
          TB421 Anti-Cre Antibody