PC645 Anti-Cleaved Bid (Ab-1) Rabbit pAb

PC645
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC645-10T
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 10 t
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          Description
          OverviewRecognizes the ~15 kDa cleaved Bid protein. Does not recognize uncleaved, full-length Bid.
          Catalogue NumberPC645
          Brand Family Calbiochem®
          Application Data
          Detection of mouse cleaved Bid by immunoblotting. Samples: Whole cell lysate from L929 cells left untreated (lane 1), treated with actinomycin alone (5 µg/ml, lane 2), or treated with 5 µg/ml actinomycin plus 2.5 ng/ml, 5 ng/ml, and 10 ng/ml mouse TNFα (lanes 3, 4, 5, respectively). Primary antibody: Anti-Cleaved Bid (Ab-1) Rabbit pAb (Cat. No. PC645) (right panel) (0.5 µg/ml) and Anti-Bid total left panel (5 µg/ml). Detection: chemiluminescence.
          References
          ReferencesEskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
          Chou, J.J., et al. 1999. Cell 96, 615.
          Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
          Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
          Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
          Luo, X., et al. 1999. Cell 94, 481.
          Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
          Footz, T.K., et al. 1998. Genomics 51, 472.
          Wang, K., et al. 1996. Genes Dev. 10, 2859.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg+2 and Ca+2), 1 mg/ml BSA, 50% glycerol, pH 7.3.
          Positive controlTNF-α-treated L929 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000, chemiluminescence)
          Application CommentsAt a 1:1000 dilution there is sufficient antibody to perform 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids at the N-terminus of the 59/60 cleavage site of mouse Bid (BH3 interacting domain death agonist)
          ImmunogenMouse
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mouse
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Cleaved Bid (Ab-1) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Cleaved Bid (Ab-1) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC645

          References

          Reference overview
          Eskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
          Chou, J.J., et al. 1999. Cell 96, 615.
          Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
          Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
          Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
          Luo, X., et al. 1999. Cell 94, 481.
          Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
          Footz, T.K., et al. 1998. Genomics 51, 472.
          Wang, K., et al. 1996. Genes Dev. 10, 2859.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-April-2009 RFH
          ApplicationImmunoblotting (1:1000, chemiluminescence)
          Application Data
          Detection of mouse cleaved Bid by immunoblotting. Samples: Whole cell lysate from L929 cells left untreated (lane 1), treated with actinomycin alone (5 µg/ml, lane 2), or treated with 5 µg/ml actinomycin plus 2.5 ng/ml, 5 ng/ml, and 10 ng/ml mouse TNFα (lanes 3, 4, 5, respectively). Primary antibody: Anti-Cleaved Bid (Ab-1) Rabbit pAb (Cat. No. PC645) (right panel) (0.5 µg/ml) and Anti-Bid total left panel (5 µg/ml). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~15 kDa cleaved mouse Bid.
          BackgroundBid (~22 kDa) is a BH3 domain containing pro-apoptotic cytosolic protein that maps to human chromosome 22q11.2. Initially localized to the cytoplasm, the Bid protein is cleaved by the action of caspase 8 into fragments of two different sizes, p15 and p13 and becomes inserted into the mitochondrial membrane. Of the two fragments the p15 BH3 containing fragment is the more potent inducer of apoptosis and in fact is a stronger inducer than the full-length Bid protein. Following cleavage by caspase 8, Bid will bind with Bax localizing it to the mitochondrial membrane and leading to the release of cyto-chrome c. This interaction is blocked by Bcl-x, which directly binds to Bax, competing for the binding of Bax to the membrane-localized Bid.
          HostRabbit
          Immunogen speciesMouse
          Immunogena synthetic peptide corresponding to amino acids at the N-terminus of the 59/60 cleavage site of mouse Bid (BH3 interacting domain death agonist)
          IsotypeIgG
          Speciesnot human, mouse
          Positive controlTNF-α-treated L929 cells
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg+2 and Ca+2), 1 mg/ml BSA, 50% glycerol, pH 7.3.
          Preservative≤0.1% sodium azide
          CommentsAt a 1:1000 dilution there is sufficient antibody to perform 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesEskes, R., et al. 2000. Mol. Cell. Biol. 20, 929.
          Chou, J.J., et al. 1999. Cell 96, 615.
          Desagher, S., et al. 1999. J. Cell Biol. 144, 891.
          Gross, A., et al. 1999. J. Biol. Chem. 274, 1156.
          Kluck, R.M., et al. 1999. J. Cell Biol. 147, 809.
          Luo, X., et al. 1999. Cell 94, 481.
          Tan, K.O., et al. 1999. J. Biol. Chem 274, 23687.
          Footz, T.K., et al. 1998. Genomics 51, 472.
          Wang, K., et al. 1996. Genes Dev. 10, 2859.