PC679 Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb

PC679
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC679-50UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 50 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~17 kDa cleaved fragment of caspase-3.
          Catalogue NumberPC679
          Brand Family Calbiochem®
          SynonymsAnti-Active Caspase-3
          Application Data
          Detection of human cleaved caspase-3 by immunoblotting. Sample: Whole cell lysate from Jurkat cells treated with camptothecin (2 µM). Primary antibody: Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC679). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationIn PBS, 30% glycerol, 0.5% BSA.
          Positive controlA-431 cells treated with an inducer of apoptosis
          Preservative0.01% thimerosal
          Applications
          Key Applications Paraffin Sections
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesFrozen Sections (10-20 µg/ml)
          Immunoblotting (0.5-1 µg/ml)
          Immunocytochemistry (10-20 µg/ml)
          Paraffin Sections (10-20 µg/ml, heat pre-treatment required)
          Application CommentsInduction of caspase-3 can occur with many different treatments, such as treatment with 0.5 µg/ml actinomycin D for 24 h. Useful for the identification of apoptotic cells in tissue sections and populations of cells. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids surrounding the Asp¹⁷⁵ cleavage site of caspase-3
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC679
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision29-September-2016 JSW
          SynonymsAnti-Active Caspase-3
          ApplicationFrozen Sections (10-20 µg/ml)
          Immunoblotting (0.5-1 µg/ml)
          Immunocytochemistry (10-20 µg/ml)
          Paraffin Sections (10-20 µg/ml, heat pre-treatment required)
          Application Data
          Detection of human cleaved caspase-3 by immunoblotting. Sample: Whole cell lysate from Jurkat cells treated with camptothecin (2 µM). Primary antibody: Anti-Caspase-3, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC679). Detection: chemiluminescence.
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~17 kDa cleaved fragment of caspase-3.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids surrounding the Asp¹⁷⁵ cleavage site of caspase-3
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlA-431 cells treated with an inducer of apoptosis
          FormLiquid
          FormulationIn PBS, 30% glycerol, 0.5% BSA.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative0.01% thimerosal
          CommentsInduction of caspase-3 can occur with many different treatments, such as treatment with 0.5 µg/ml actinomycin D for 24 h. Useful for the identification of apoptotic cells in tissue sections and populations of cells. Antibody should be titrated for optimal results in individual systems.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling