208912 | Anti-Calreticulin Mouse mAb (FMC75)

208912
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, Mk M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      208912-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
          Catalogue Number208912
          Brand Family Calbiochem®
          Application Data
          Detection of human calreticulin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1) and Vero cells (lane 2). Primary antibody: Anti-Calreticulin Mouse mAb (FMC75) (Cat. No. 208912) (1:1000). Detection: chemiluminescence.
          References
          ReferencesNauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
          Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
          Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
          Product Information
          FormLiquid
          FormulationIn PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
          Positive controlHuman Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
          PreservativeNone
          Applications
          Application ReferencesImmunoblotting, Flow Cytometry Broquet, A.H., et al. 2003. J. Biol. Chem. 278, 21601. Immunocytochemistry Chen, A., et al. 2002. Biochim. Biophys. Acta 1589, 124. Morello, J.P., et al. 2000. J. Clin. Invest. 105, 887. Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150. Immunohistochemistry Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150.
          Key Applications Immunocytochemistry
          Immunohistochemistry
          Enzyme Immunoassay
          Flow Cytometry
          Immunoblotting (Western Blotting)
          Application NotesEnzyme Immunoassay (0.1 µg/ml)
          Immunoblotting (0.5 µg/ml, colorimetric; see application references)
          Immunocytochemistry (see application references)
          Immunohistochemistry (see application references)
          Flow Cytometry (see application references)
          Application CommentsDoes not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena recombinant protein consisting of human calreticulin fused to maltose binding protein
          ImmunogenHuman
          CloneFMC75
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Monkey
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          208912

          References

          Reference overview
          Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
          Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
          Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-June-2008 JSW
          ApplicationEnzyme Immunoassay (0.1 µg/ml)
          Immunoblotting (0.5 µg/ml, colorimetric; see application references)
          Immunocytochemistry (see application references)
          Immunohistochemistry (see application references)
          Flow Cytometry (see application references)
          Application Data
          Detection of human calreticulin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1) and Vero cells (lane 2). Primary antibody: Anti-Calreticulin Mouse mAb (FMC75) (Cat. No. 208912) (1:1000). Detection: chemiluminescence.
          DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
          BackgroundCalreticulin is a calcium binding protein found primarily in the ER and the sarcoplasmic reticulum, although it also has been detected in the nucleus and nuclear envelope. It has the conserved KDEL sequence at its C-terminus for retention in the ER. It contains both high and low affinity calcium binding sites, is involved in calcium storage and regulation. The calcium binding at the C-terminus of calreticulin has been postulated to be involved with regulating the interaction of KDEL proteins with the KDEL receptor. Calreticulin also acts as a molecular chaperone for protein folding, in concert with Grp78 and Grp94.
          HostMouse
          Immunogen speciesHuman
          Immunogena recombinant protein consisting of human calreticulin fused to maltose binding protein
          CloneFMC75
          IsotypeIgG₁
          Speciesnot E. coli, not bovine, not canine, not guinea pig, not hamster, human, monkey, not mouse, not rat, not salmon
          Positive controlHuman Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
          FormLiquid
          FormulationIn PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsDoes not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesNauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
          Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
          Smith, M.J., and Koch, G.L.E. 1989. EMBO J. 8, 3581.
          Application referencesImmunoblotting, Flow Cytometry Broquet, A.H., et al. 2003. J. Biol. Chem. 278, 21601. Immunocytochemistry Chen, A., et al. 2002. Biochim. Biophys. Acta 1589, 124. Morello, J.P., et al. 2000. J. Clin. Invest. 105, 887. Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150. Immunohistochemistry Cavill, D., et al. 1999. Applied Immunohistochemistry & Molecular Morphology 7, 150.