208880 | Anti-Calnexin, C-Terminal (575-593) Rabbit pAb

208880
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      B, Ca, Gp, Ht, H, Mk, M, Rb, R, Sh Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      208880-50UL
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          Plastic ampoule 50 ul
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          Description
          OverviewRecognizes the ~90 kDa calnexin protein.
          Catalogue Number208880
          Brand Family Calbiochem®
          SynonymsAnti-IP90, Anti-p88
          Application Data
          Detection of human calnexin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1), Vero (lane 2), Rat-2 cells (lane 3) and L-929 cells (lane 4). Primary antibody: Anti-Calnexin, C-Terminal (575-593) Rabbit pAb (Cat. No. 208880) (1:1000). Detection: chemiluminescence.
          References
          ReferencesHammond, C., et al. 1994. J. Cell Biol. 126, 41.
          Ou, W.-J., et al. 1993. Nature 364, 771.
          David, V., et al. 1993. J. Biol. Chem. 268, 9585.
          Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
          Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
          Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
          Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Positive controlHeat-shocked HeLa cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1 µg/ml)
          Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide (AEEDEILNRSPRNRKPRRE) corresponding to amino acids 575-593 of canine calnexin, conjugated to KLH
          ImmunogenCanine
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Bovine
          • Canine
          • Guinea Pig
          • Hamster
          • Human
          • Monkey
          • Mouse
          • Rabbit
          • Rat
          • Sheep
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          208880

          References

          Reference overview
          Hammond, C., et al. 1994. J. Cell Biol. 126, 41.
          Ou, W.-J., et al. 1993. Nature 364, 771.
          David, V., et al. 1993. J. Biol. Chem. 268, 9585.
          Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
          Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
          Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
          Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision31-January-2008 JSW
          SynonymsAnti-IP90, Anti-p88
          ApplicationImmunoblotting (1 µg/ml)
          Application Data
          Detection of human calnexin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1), Vero (lane 2), Rat-2 cells (lane 3) and L-929 cells (lane 4). Primary antibody: Anti-Calnexin, C-Terminal (575-593) Rabbit pAb (Cat. No. 208880) (1:1000). Detection: chemiluminescence.
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~90 kDa calnexin protein.
          BackgroundCalnexin is an ER transmembrane protein that transiently associates with newly synthesized, incompletely folded monomeric glycoproteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRµ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
          HostRabbit
          Immunogen speciesCanine
          Immunogena synthetic peptide (AEEDEILNRSPRNRKPRRE) corresponding to amino acids 575-593 of canine calnexin, conjugated to KLH
          IsotypeIgG
          Speciesbovine, canine, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep
          Positive controlHeat-shocked HeLa cells
          FormLiquid
          FormulationIn PBS, 50% glycerol, pH 7.2.
          Preservative≤0.1% sodium azide
          CommentsVariables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesHammond, C., et al. 1994. J. Cell Biol. 126, 41.
          Ou, W.-J., et al. 1993. Nature 364, 771.
          David, V., et al. 1993. J. Biol. Chem. 268, 9585.
          Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
          Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
          Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
          Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.