PC205L Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb

PC205L
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC205L-100UL
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the α-calcitonin gene related peptide in rat amygdala and spinal cord.
          Catalogue NumberPC205L
          Brand Family Calbiochem®
          SynonymsAnti-CGRP
          Application Data
          Detection of rat calcitonin gene related peptide by immunohistochemistry. Sample: Rat amygdala and spinal cord. Primary antibody: Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb (Cat. No. PC205L) (1:400). Detection: fluorescence.
          References
          ReferencesSudhof, T.C. 1995. Nature 375, 645.
          Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
          Product Information
          FormLyophilized
          FormulationUndiluted serum.
          Positive controlRat amygdala or spinal cord
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesParaffin Sections, Free Floating Sections, Immunofluorescence Maatkamp, A., et al. 2004. Eur. J. Neurosci. 20, 14.
          Key Applications Paraffin Sections
          Free-floating Sections
          Frozen Sections
          Immunofluorescence
          Application NotesFree Floating Sections (see application references)
          Frozen Sections (1:2000-1:4000, ABC detection)
          Immunofluorescence (1:100-1:200, see application references)
          Paraffin Sections (see application references; heat pre-treatment required; see comments)
          Application CommentsAntibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrat α-calcitonin gene related peptide (α-CGRP)
          ImmunogenRat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC205L

          References

          Reference overview
          Sudhof, T.C. 1995. Nature 375, 645.
          Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision09-October-2007 RFH
          SynonymsAnti-CGRP
          ApplicationFree Floating Sections (see application references)
          Frozen Sections (1:2000-1:4000, ABC detection)
          Immunofluorescence (1:100-1:200, see application references)
          Paraffin Sections (see application references; heat pre-treatment required; see comments)
          Application Data
          Detection of rat calcitonin gene related peptide by immunohistochemistry. Sample: Rat amygdala and spinal cord. Primary antibody: Anti-Calcitonin Gene Related Peptide (CGRP) Rabbit pAb (Cat. No. PC205L) (1:400). Detection: fluorescence.
          DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the α-CGRP protein.
          BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. The calcitonin gene peptide superfamily consists of calcitonin, calcitonin gene-related peptide (CGRP), amylin, and adrenomedullin. CGRP is a 37 amino acid neuropeptide that shows widespread distribution and acts as a potent vasodilator. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
          HostRabbit
          Immunogen speciesRat
          Immunogenrat α-calcitonin gene related peptide (α-CGRP)
          IsotypeIgG
          Speciesmouse, rat
          Positive controlRat amygdala or spinal cord
          FormLyophilized
          FormulationUndiluted serum.
          Preservative≤0.1% sodium azide
          CommentsAntibody specificity was examined in the rat amygdala and spinal cord. Staining is completely eliminated by pre-treatment of antibody with 10-5M rat α-CGRP and partially eliminated by preabsorption with rat β-CGRP. Preabsorption with calcitonin resulted in no loss of immunostaining. For staining paraffin sections, heat sections to 80°C in 40 mM sodium citrate buffer, pH 8.5. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Carefully reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Avoid freeze/thaw cycles of solutions.
          Toxicity Standard Handling
          ReferencesSudhof, T.C. 1995. Nature 375, 645.
          Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
          Application referencesParaffin Sections, Free Floating Sections, Immunofluorescence Maatkamp, A., et al. 2004. Eur. J. Neurosci. 20, 14.