217590 Anti-CD40 Mouse mAb (EA-5)

217590
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      217590-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ~44 kDa CD40 protein in Raji cells.
          Catalogue Number217590
          Brand Family Calbiochem®
          References
          ReferencesFanslow, W.C., et al. 1992. J. Immunol. 149, 655.
          Law, C.L., et al. 1990. Leukemia 4, 732.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.
          Positive controlRaji cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Paraffin Sections
          Flow Cytometry
          Frozen Sections
          Immunoprecipitation
          Application NotesFlow cytometry (5 µg/ml)
          Frozen Sections (see comments)
          Immunoprecipitation (see comments)
          Paraffin Sections (see comments)
          Application CommentsFACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogentransfected murine L cells expressing human CD40
          ImmunogenHuman
          CloneEA-5
          HostMouse
          Purity≥95% by SDS-PAGE
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          217590

          References

          Reference overview
          Fanslow, W.C., et al. 1992. J. Immunol. 149, 655.
          Law, C.L., et al. 1990. Leukemia 4, 732.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-December-2008 JSW
          ApplicationFlow cytometry (5 µg/ml)
          Frozen Sections (see comments)
          Immunoprecipitation (see comments)
          Paraffin Sections (see comments)
          DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~44 kDa CD40 protein.
          BackgroundHuman CD40 is a member of the tumor necrosis factor (TNF) family of cell surface receptors, and is found on all B cells (except plasma cells) and some epithelial, carcinoma, and lymphoid dendritic cells. Through binding of its ligand (CD40L/CD154/gp39), CD40 plays an important role in B-cell activation and isotype switching.
          HostMouse
          Immunogen speciesHuman
          Immunogentransfected murine L cells expressing human CD40
          CloneEA-5
          IsotypeIgG₁
          Specieshuman
          Positive controlRaji cells
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 0.5 mg/ml gentamicin sulfate, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥95% by SDS-PAGE
          Preservative≤0.1% sodium azide
          CommentsFACS was performed on 5x105 cultured Raji human tumor cells. Optimal binding concentration tested was 5 µg/ml using FITC-conjugated goat anti-mouse IgG as a secondary antibody. This antibody has also been reported to work for frozen sections, immunoprecipitation, and paraffin sections. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesFanslow, W.C., et al. 1992. J. Immunol. 149, 655.
          Law, C.L., et al. 1990. Leukemia 4, 732.