217570 | Anti-CD3 Mouse mAb (UCHT1)

217570
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      217570-100UG
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes the ε-chain of the CD3 complex in human peripheral T cells. Can be used to activate T cells expressing CD3ε.
          Catalogue Number217570
          Brand Family Calbiochem®
          References
          ReferencesThibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
          Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.
          Product Information
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Preservative0.5 mg/ml gentamycin sulfate
          Applications
          Application ReferencesT cell activation Lafont, V., et al. 1999. J. Biol. Chem. 274, 25743.
          Key Applications T Cell Co-stimulation
          Flow Cytometry
          Frozen Sections
          Immunoprecipitation
          Application NotesFlow Cytometry (5 µg/ml)
          Frozen Sections (see comments)
          Immunoprecipitation (see comments)
          T cell Co-stimulation (see application references)
          Application CommentsThis antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenhuman thymocytes/Sezary T cells
          ImmunogenHuman
          CloneUCHT1
          HostMouse
          Purity>95% immunoglobulin by SDS-PAGE
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          217570

          References

          Reference overview
          Thibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
          Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.

          Citations

          Title
        • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision25-July-2007 RFH
          ApplicationFlow Cytometry (5 µg/ml)
          Frozen Sections (see comments)
          Immunoprecipitation (see comments)
          T cell Co-stimulation (see application references)
          DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~20 kDa ε chain of the CD3 T-cell receptor complex.
          HostMouse
          Immunogen speciesHuman
          Immunogenhuman thymocytes/Sezary T cells
          CloneUCHT1
          IsotypeIgG₁
          Specieshuman
          FormLiquid
          FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity>95% immunoglobulin by SDS-PAGE
          Preservative0.5 mg/ml gentamycin sulfate
          CommentsThis antibody can be used to activate T cells expressing CD3ε (see application reference). FACS was performed on 5 x 105 cultured HPB-MLT human tumor cells. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody is reported to work for immunoprecipitation and for staining frozen sections. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
          Toxicity Standard Handling
          ReferencesThibault, G. and Bardos, P. 1995. J. Immunol. 154, 3814.
          Beverley, P.C., and Callard, R.E. 1981. Eur. J. Immunol. 11, 329.
          Citation
        • Renee M. Marshall, et al. (2005) Cyclin T1 expression is regulated by multiple signaling pathways and mechanisms during activation of human peripheral blood lymphocytes. Journal of Immunology 175, 6402-6411.
        • Application referencesT cell activation Lafont, V., et al. 1999. J. Biol. Chem. 274, 25743.