ABC253 Anti-Beclin-2

ABC253
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H WB, IH(P) Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberABC253
      DescriptionAnti-Beclin-2
      Alternate Names
      • Beclin2
      Background InformationBeclin-2 (UniProt: P0DM65) is encoded by Becn2 gene (Gene ID: 226720) in murine species. Beclin-2 is a member of the Atg6/Beclin family and is involved in 2 distinct lysosomal degradation pathways and acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. It regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1. Beclin-2 family members are specific to mammals whereas Becln-1 subfamily members are present in more primitive vertebrates. Beclin-2 interacts with ATG14, AMBRA1, UVRAG and PIK3C3/VPS34 and this interaction is not disrupted by starvation. It is expressed in brain, skeletal muscle, placenta, thymus and uterus and expression is lower in liver, testis, stomach, and 17-day-old embryos. Disruption of Beclin-2 is shown to reduce embryonic viability and heterozygous knockout mice show defects in autophagy, increased levels of Cnr1 receptor, elevated food intake, and obesity and insulin resistance. (Ref.: He, C. et al. (2013). Cell 154, 1085-1099).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Beclin-2 Antibody, Cat. No. ABC253, is a rabbit polyclonal antibdoy that detects Beclin-2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Beclin-2 in human brain (thalamic region) and mouse stomach tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of murine Beclin-2.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Becline-2 in murine cells. It targets an epitope within 14 amino acids from the N-terminal.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Becn2
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~50 kDa observed; 50.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Beclin2 +/+ Mouse Brain (Wild-Type).

      Western Blotting Analysis: 2 µg/mL of this antibody detected Beclin-2 in lysate from Beclin-2 +/+ Mouse Brain (Wild-type).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications