MABC34 Anti-Beclin-1 Antibody, clone 9A1.1

MABC34
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P), FC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC34
      ReplacesMABN16
      DescriptionAnti-Beclin-1 Antibody, clone 9A1.1
      Alternate Names
      • Beclin-1
      • Coiled-coil myosin-like BCL2-interacting protein
      • Protein GT197
      Background InformationThe Beclin-1 (BECN1) gene encodes a 60 kDa coiled-coil protein that interacts with the prototypic apoptosis inhibitor Bcl-2 (1). Beclin-1 promotes autophagy in autophagy-defective yeast with a targeted disruption of agp6/vps30, and in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin-1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation, in vitro clonigenicity, tumorigenesis, and has been found to ameliorate the neurodegenerative pathology in α-Synuclein models of Parkinson's and lewy body diseases. Endogenous Beclin-1 protein expression is frequently low in human breast epithelial carcinoma cell lines and tissue, but is expressed ubiquitously at high levels in normal breast epithelia (2). It has also been shown that Beclin 1 contains a leucine-rich nuclear export signal motif raising the possibility that its autophagy and/or tumor suppressor function may require regulated, CRM1-dependent, nucleocytoplasmic transport (3).
      References
      Product Information
      FormatPurified
      Control
      • Raji cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Beclin-1 Antibody, clone 9A1.1 detects level of Beclin-1 & has been published & validated for use in Western Blotting, IHC(P), Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Beclin-1 in human colorectal adenocarcinoma tissue.

      Flow Cytometry Analysis: 1 µg from a representative lot detected Beclin-1 in HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Beclin-1.
      Clone9A1.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BECN1
      • GT197
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~54 kDa kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Raji cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Beclin-1 in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Beclin-1 Antibody, clone 9A1.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Beclin-1 Antibody, clone 9A1.1 Certificates of Analysis

      TitleLot Number
      Anti-Beclin-1, clone 9A1.1 (mouse monoclonal) Monoclonal AntibodyQ2925514
      Anti-Beclin-1, clone 9A1.1 - 24479672447967
      Anti-Beclin-1, clone 9A1.1 - 24521652452165
      Anti-Beclin-1, clone 9A1.1 - 24688172468817
      Anti-Beclin-1, clone 9A1.1 - 25185742518574
      Anti-Beclin-1, clone 9A1.1 - 32595263259526
      Anti-Beclin-1, clone 9A1.1 - Q2035683Q2035683
      Anti-Beclin-1, clone 9A1.1 -26896562689656
      Anti-Beclin-1, clone 9A1.1 -28202482820248
      Anti-Beclin-1, clone 9A1.1 Monoclonal Antibody3103638

      References

      Reference overviewPub Med ID
      Microglia change from a reactive to an age-like phenotype with the time in culture.
      Caldeira, C; Oliveira, AF; Cunha, C; Vaz, AR; Falcão, AS; Fernandes, A; Brites, D
      Frontiers in cellular neuroscience  8  152  2014

      Show Abstract
      24917789 24917789

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies