178613 Anti-Aquaporin 3 (275-292) Rabbit pAb

178613
  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      R Rb Polyclonal Antibody
      Description
      OverviewRecognizes the ~40 kDa and ~29 kDa forms of aquaporin-3 in rat kidney membrane. Supplied with a control peptide (immunogen).
      Catalogue Number178613
      Brand Family Calbiochem®
      SynonymsAnti-AQP3, Anti-GLIP
      Application Data
      Detection of rat aquaporin 3 by immunoblotting. Sample: Extract from rat kidney membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (1:200). Detection: chemiluminescence.

      Detection of rat aquaporin 3 by immunohistochemistry. Samples: Longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (Dilution Assay Dependent). Detection: fluorescence.
      References
      ReferencesEchevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
      Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
      Product Information
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, 5% sucrose, pH. 7.4 and 40 µg AQP3275-292 control peptide lyophilized from PBS.
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunohistochemistry Ishibashi, K., et al. 1997. Am. J. Physiol. 272, F235. Ecelbarger, C.A., et al. 1995. Am. J. Physiol. 269, F663.
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (1:200)
      Immunohistochemistry (assay dependent; see application references)
      Application CommentsFor use as a negative control, preincubate 1 µg of peptide with 1 µg of antibody for 1 h at room temperature. The epitope is unique for AQP3 and is not present in other known related proteins. It is highly homologous in mouse (17/18 identical) and human (15/18 identical) AQP3 protein. Recognition of AQP3 can be fully inhibited with the soluble AQP3275-292 blocking peptide. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide [(KY)STEAENVKLAH(Nle)KHKEQI] corresponding to amino acids 275-292 of rat AQP3, with norleucine replacing methionine, conjugated to KLH
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 50 µl deionized water to make a 300 µg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Reconstitue the peptide with 100 µl deionized water, aliquot, and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Aquaporin 3 (275-292) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Aquaporin 3 (275-292) Rabbit pAb Certificates of Analysis

      TitleLot Number
      178613

      References

      Reference overview
      Echevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
      Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-November-2009 RFH
      SynonymsAnti-AQP3, Anti-GLIP
      ApplicationImmunoblotting (1:200)
      Immunohistochemistry (assay dependent; see application references)
      Application Data
      Detection of rat aquaporin 3 by immunoblotting. Sample: Extract from rat kidney membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (1:200). Detection: chemiluminescence.

      Detection of rat aquaporin 3 by immunohistochemistry. Samples: Longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME). Primary antibody: Anti-Aquaporin 3 (275-292) Rabbit pAb (Cat. No. 178613) (Dilution Assay Dependent). Detection: fluorescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40 kDa and ~29 kDa aquaporin-3 proteins. Supplied with a control peptide (immunogen).
      BackgroundAQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It is also slightly permeable to urea and glycerol. AQP3 may play an important role in gastrointestinal tract water transport and in glycerol metabolism. AQP3 has a wide tissue distribution. In the kidney it is predominantly expressed in the inner medulla.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide [(KY)STEAENVKLAH(Nle)KHKEQI] corresponding to amino acids 275-292 of rat AQP3, with norleucine replacing methionine, conjugated to KLH
      IsotypeIgG
      Speciesrat
      FormLyophilized
      Formulation50 µl antibody lyophilized from PBS, 1% BSA, 5% sucrose, pH. 7.4 and 40 µg AQP3275-292 control peptide lyophilized from PBS.
      Preservative≤0.1% sodium azide
      CommentsFor use as a negative control, preincubate 1 µg of peptide with 1 µg of antibody for 1 h at room temperature. The epitope is unique for AQP3 and is not present in other known related proteins. It is highly homologous in mouse (17/18 identical) and human (15/18 identical) AQP3 protein. Recognition of AQP3 can be fully inhibited with the soluble AQP3275-292 blocking peptide. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 50 µl deionized water to make a 300 µg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Reconstitue the peptide with 100 µl deionized water, aliquot, and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesEchevarria, M., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 10997.
      Ishibashi, K., et al. 1994. Proc. Natl. Acad. Sci. USA 91, 6269.
      Application referencesImmunohistochemistry Ishibashi, K., et al. 1997. Am. J. Physiol. 272, F235. Ecelbarger, C.A., et al. 1995. Am. J. Physiol. 269, F663.