178612 | Anti-Aquaporin 2 (254-271) Rabbit pAb

178612
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      178612-1ST
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          Plastic ampoule 1 st
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          Description
          OverviewRecognizes the ~40 kDa and ~29 kDa forms of aquaporin-2 in rat kidney membrane. Supplied with a control peptide (AQP2254-271 immunogen peptide).
          Catalogue Number178612
          Brand Family Calbiochem®
          SynonymsAnti-AQP2, Anti-AQP-CD, Anti-WCH-CD
          Application Data
          Detection of rat aquaporin 2 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with a control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:200). Detection: chemiluminescence.

          Detection of rat aquaporin 2 by immunohistochemistry. Sample: Rat kidney showing the inner medulla. Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:100). Detection: fluorescence.
          References
          ReferencesFushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
          Fushimi, K., et al. 1993. Nature 361, 549.
          Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654.
          Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
          Product Information
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40 µg control peptide lyophilized from PBS.
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoprecipitation Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800. Immunohistochemistry Fushimi, K., et al. 1993. Nature 361, 549. Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
          Key Applications Immunoblotting (Western Blotting)
          Immunohistochemistry
          Immunoprecipitation
          Application NotesImmunoblotting (1:200-1:600)
          Immunohistochemistry (see application reference)
          Immunoprecipitation (see application reference)
          Application CommentsImmunogen peptide is identical in rat and mouse protein, and differs by one amino acid in the human sequence. The epitope is unique for AQP2 and is not present in other known related proteins. It is identical in mouse and rat, and only slightly different (17/18 identical) in human. To prepare a negative control, preincubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide [(KY)RQSVELHSPQSLPRGSKA] corresponding to amino acids 254-271 of rat and mouse AQP2, conjugated to KLH
          ImmunogenMouse/Rat
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsPlease see antibody label for lot specific reconstitution instructions. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the peptide in 100 µl deionized water, aliquot, and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          178612

          References

          Reference overview
          Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
          Fushimi, K., et al. 1993. Nature 361, 549.
          Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654.
          Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-February-2008 JSW
          SynonymsAnti-AQP2, Anti-AQP-CD, Anti-WCH-CD
          ApplicationImmunoblotting (1:200-1:600)
          Immunohistochemistry (see application reference)
          Immunoprecipitation (see application reference)
          Application Data
          Detection of rat aquaporin 2 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with a control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:200). Detection: chemiluminescence.

          Detection of rat aquaporin 2 by immunohistochemistry. Sample: Rat kidney showing the inner medulla. Primary antibody: Anti-Aquaporin 2 (254-271) Rabbit pAb (Cat. No. 178612) (1:100). Detection: fluorescence.
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~40 kDa and ~29 kDa aquaporin-2 proteins. Supplied with a control peptide (AQP2254-271 immunogen peptide).
          BackgroundAQP2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. Defects in AQP2 are a cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI).
          HostRabbit
          Immunogen speciesMouse/Rat
          Immunogena synthetic peptide [(KY)RQSVELHSPQSLPRGSKA] corresponding to amino acids 254-271 of rat and mouse AQP2, conjugated to KLH
          IsotypeIgG
          Specieshuman, mouse, rat
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, 5% sucrose, pH 7.4 and 40 µg control peptide lyophilized from PBS.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsImmunogen peptide is identical in rat and mouse protein, and differs by one amino acid in the human sequence. The epitope is unique for AQP2 and is not present in other known related proteins. It is identical in mouse and rat, and only slightly different (17/18 identical) in human. To prepare a negative control, preincubate 1 µg peptide with 1 µg antibody for 1 h at room temperature. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsPlease see antibody label for lot specific reconstitution instructions. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Following reconstitution, aliquot and freeze (-20°C). Reconstitute the peptide in 100 µl deionized water, aliquot, and freeze (-20°C).
          Toxicity Irritant
          ReferencesFushimi, K., et al. 1997. J. Biol. Chem. 272, 14800.
          Fushimi, K., et al. 1993. Nature 361, 549.
          Ma, T., et al. 1993. Biochem. Biophys. Res. Commun. 197, 654.
          Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.
          Application referencesImmunoprecipitation Fushimi, K., et al. 1997. J. Biol. Chem. 272, 14800. Immunohistochemistry Fushimi, K., et al. 1993. Nature 361, 549. Nielson, S., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 11663.