178611 | Anti-Aquaporin 1 (243-261) Rabbit pAb

178611
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      178611-1ST
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          Plastic ampoule 1 st
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          Description
          OverviewRecognizes aquaporin-1 in red blood cells. Supplied with AQP1243-261 blocking peptide (immunogen).
          Catalogue Number178611
          Brand Family Calbiochem®
          SynonymsAnti-AQP1, Anti-CHIP28
          Application Data
          Detection of rat aquaporin 1 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with the control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 1 (243-261) Rabbit pAb (Cat. No. 179611) (1:400). Detection: chemiluminescence.
          References
          ReferencesKing, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
          Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
          Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.
          Product Information
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, pH 7.4 and 40 µg blocking peptide lyophilized from PBS.
          Preservative≤0.1% sodium azide (antibody only)
          Applications
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
          Frozen Sections (see comments)
          Application CommentsThe epitope is unique for AQP1 and is not present in other known related proteins. It is identical in human, rodent, and cattle, and only slightly different in frog. Recognition of AQP1 can be fully inhibited with the soluble AQP1243-261 blocking peptide. For use as a negative control, preincubate 1 µg blocking peptide with 1 µg antibody for 1 h at room temperature. This antibody is also reported to work with frozen sections. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic peptide (KVWTSGQVEEYDLDADDIN) corresponding to amino acids 243-261 of human AQP1, conjugated to KLH
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsDissolve the antibody in 50 µl deionized water to make a 1 mg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of stock solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Dissolve the peptide in 100 µl deionized water and store in aliquots at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          178611

          References

          Reference overview
          King, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
          Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
          Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-June-2007 RFH
          SynonymsAnti-AQP1, Anti-CHIP28
          ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
          Frozen Sections (see comments)
          Application Data
          Detection of rat aquaporin 1 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with the control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 1 (243-261) Rabbit pAb (Cat. No. 179611) (1:400). Detection: chemiluminescence.
          DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the aquaporin-1 protein. Supplied with AQP1243-261 blocking peptide (immunogen).
          BackgroundAQP1 is a 269 amino acid transmembrane protein belonging to the aquaporin family. AQP1 forms the major water transports pathway of red cells and renal proximal tubules and thin descending limb of Henle, but not collecting duct.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide (KVWTSGQVEEYDLDADDIN) corresponding to amino acids 243-261 of human AQP1, conjugated to KLH
          IsotypeIgG
          Specieshuman, rat
          FormLyophilized
          Formulation50 µl antibody lyophilized from PBS, pH 7.4 and 40 µg blocking peptide lyophilized from PBS.
          Preservative≤0.1% sodium azide (antibody only)
          CommentsThe epitope is unique for AQP1 and is not present in other known related proteins. It is identical in human, rodent, and cattle, and only slightly different in frog. Recognition of AQP1 can be fully inhibited with the soluble AQP1243-261 blocking peptide. For use as a negative control, preincubate 1 µg blocking peptide with 1 µg antibody for 1 h at room temperature. This antibody is also reported to work with frozen sections. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsDissolve the antibody in 50 µl deionized water to make a 1 mg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of stock solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Dissolve the peptide in 100 µl deionized water and store in aliquots at -20°C.
          Toxicity Standard Handling
          ReferencesKing, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
          Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
          Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.