176002 Anti-Angiopoietin-2 Rabbit pAb

176002
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      176002-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecognizes both the ~55 kDa non-glycosylated and the ~70 kDa glycosylated, secreted Ang-2 protein in cell culture medium. Does not cross-react with Ang-1.
        • Antibody Target Gene Symbol: ANGPT2
        • Target Synonym: AGPT2, ANG II, ANG-2, ANGIOPOIETIN, ANGIOPOIETIN 2, Angiotensin 2, Angrp
        • Entrez Gene Name: angiopoietin 2
        • Hu Entrez ID: 285
        • Mu Entrez ID: 11601
        • Rat Entrez ID: 89805
        • Catalogue Number176002
          Brand Family Calbiochem®
          SynonymsAnti-Ang-2
          References
          ReferencesMaisonpierre, P.C., et al. 1997. Science 277, 55.
          Davis, S., et al. 1996. Cell 87, 1161.
          Suri, S. et al. 1996. Cell 87, 1171.
          Product Information
          FormLiquid
          FormulationIn PBS, 0.1% BSA, pH 7.5.
          PreservativeNone
          Applications
          Key Applications Immunohistochemistry
          Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Application NotesELISA (0.1-1 µg/ml)
          Immunoblotting (1-10 µg/ml)
          Immunofluorescence (2-20 µg/ml)
          Immunohistochemistry (2-20 µg/ml)
          Application CommentsThe immunogenic peptide sequence is 71% (15/21 amino acids) conserved in the human angiopoietin-2 protein. Does not cross-react with angiopoietin-1. Variables associated with assay conditions will dictate the optimal working dilution.
          Biological Information
          Immunogena synthetic peptide corresponding to a 21 amino acid cytoplasmic sequence at the N-terminus of mouse angiopoietin-2
          ImmunogenMouse
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mouse
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          176002

          References

          Reference overview
          Maisonpierre, P.C., et al. 1997. Science 277, 55.
          Davis, S., et al. 1996. Cell 87, 1161.
          Suri, S. et al. 1996. Cell 87, 1171.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-May-2008 JSW
          SynonymsAnti-Ang-2
          ApplicationELISA (0.1-1 µg/ml)
          Immunoblotting (1-10 µg/ml)
          Immunofluorescence (2-20 µg/ml)
          Immunohistochemistry (2-20 µg/ml)
          DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes both the ~55 kDa nonglycosylated and the ~70 kDa glycosylated forms of angiopoietin-2.
          BackgroundAngiopoietin (Ang-2) is a recently identified secreted protein that may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology. Ang-2 expression is abundant in the dorsal aorta and major aortic branches. Ang-2 transcripts in fetal liver are restricted to cells at the lumen of hepatic vessels. In adults, Ang-2 is prominently expressed in tissues that may undergo vascular remodeling (ovary, placenta, and uterus).
          HostRabbit
          Immunogen speciesMouse
          Immunogena synthetic peptide corresponding to a 21 amino acid cytoplasmic sequence at the N-terminus of mouse angiopoietin-2
          IsotypeIgG
          Speciesmouse
          FormLiquid
          FormulationIn PBS, 0.1% BSA, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          PreservativeNone
          CommentsThe immunogenic peptide sequence is 71% (15/21 amino acids) conserved in the human angiopoietin-2 protein. Does not cross-react with angiopoietin-1. Variables associated with assay conditions will dictate the optimal working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesMaisonpierre, P.C., et al. 1997. Science 277, 55.
          Davis, S., et al. 1996. Cell 87, 1161.
          Suri, S. et al. 1996. Cell 87, 1171.