MABN640 Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody

MABN640
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H DB, WB, IHC Rb Purified Monoclonal Antibody
      Description
      Catalogue NumberMABN640
      DescriptionAnti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody
      Alternate Names
      • Amyloid Beta
      • Amyloid B
      • Amyloid beta A4 protein
      • ABPP
      • APPI
      • APP
      • Alzheimer disease amyloid protein
      • Cerebral vascular amyloid peptide
      • CVAP
      • PreA4
      • Protease nexin-II
      • PN-II
      • N-APP2
      • Soluble APP-alpha
      • S-APP-alpha
      • Soluble APP-beta
      • S-APP-beta
      • C995.Beta-amyloid protein 42
      • Beta-APP42
      • Beta-amyloid protein 40
      • Beta-APP40
      • C83
      • P3(42)
      • P3(40)
      • C80
      • Gamma-secretase C-terminal fragment 59
      • Amyloid intracellular domain 59
      • AICD-59
      • AID(59)
      • Gamma-CTF(59)
      • Gamma-secretase C-terminal fragment 57
      • Amyloid intracellular domain 57
      • AICD-57
      • AID(57)
      • Gamma-CTF(57)
      • Gamma-secretase C-terminal fragment 50
      • Amyloid intracellular domain 50
      • AICD-50
      • AID(50)
      • Gamma-CTF(50)
      • C31
      Background InformationThe protein named Amyloid beta A4, or ABPP, APP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, CVAP, PreA4, or Protease nexin II (PN-II) protein and encoded by human gene A4/AD1 is a precursor to 14 peptides some of which are associated with neurological disease such as Alzheimer's Disease. In pathological conditions Amyloid beta A4 protein is the precursor for the disease associated proteins, Beta amyloid 40, and Beta amyloid 42, major components of plaques in Alzheimer’s Disease. Normally APP plays a role in neuronal development and mediates aspects of axonal transport as well as neurite outgrowth. It is also involved in copper homeostasis/oxidative stress through copper ion reduction in neurons. Amyloid Beta A4 protein is localized to the plasma membrane and Golgi complex until cleaved. Amyloid Beta A4 protein is normally expressed in all fetal tissues and in the adult, frontal cortex expression predominates although there are isoforms widely expressed in non-neuronal cells as well. Normally, amyloid beta A4 is proteolytically processed and the cleavage and secretion of fragments are non-amyloidogenic. However under presenilin/nicastrin mediated processing of the C99 fragment releases beta amyloid proteins that are associated with neurological disease. MABN640 recognizes a specific conformation of residues 2-7 of the amyloid Aß peptide sequence (AEFRHD) found in amyloid fibrils. It does not detectably react with APP, IAPP, a-synuclein or polyQ fibrils A different antibody MABN637, recognizes a specific conformation of residues 3-6 of the amyloid Aß peptide sequence (EFRH) found in amyloid fibrils. It does not recognize pyroglutamyl Aß at residue 3 (1). MABN640 displays a distinct specificity from MABN638 on dot blots of Aß preparations (1). It also reacts with polyQ40 fibrils but not monomers, indicating that it recognizes a generic, sequence independent epitope.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody is validated for use in Dot Blot and Western Blotting and Immunohistochemistry for the detection of Amyloid beta fibrils.
      Key Applications
      • Dot Blot
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 0.1 µg/mL from a representative lot detected Amyloid beta fibrils in Abeta42 aggregates (Data courtesy of Prof. C. Glabe, UCI).
      Immunohistochemistry Analysis: A representative lot detected Amyloid beta fibrils in Frontal cortex of AD patients (Data courtesy of Prof. C. Glabe, UCI).
      Biological Information
      ImmunogenCorresponding to the Beta Amyloid Protein 42 (residue 2-7) of human Amyloid beta fibrils.
      EpitopeBeta Amyloid Protein 42 (residue 2-7)
      CloneM98
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityMABN640 recognizes a specific conformation of residues 2-7 of the amyloid Aß peptide sequence (AEFRHD) found in amyloid fibrils. It does not detectably react with APP, IAPP, a-synuclein or polyQ fibrils (Hatami et al., manuscript in preparation). This antibody reconizes both Abeta40 and ABeta42.
      IsotypeIgGκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rabbit, Bovine, Chicken based on 100% sequence homology. Other homologies: Broad species cross-reactivity is expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodProtein A Purfied
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in Synthetic Abeta40.

      Dot Blot Analysis: 0.1 µg/mL of this antibody detected Amyloid beta fibrils in 0.075 µg of Synthetic Abeta40.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal Antibody Certificates of Analysis

      TitleLot Number
      Anti-Amyloid beta fibrils, clone M98, Rabbit Monoclonal - Q2437459Q2437459

      References

      Reference overviewPub Med ID
      Positron emission tomography imaging of fibrillar parenchymal and vascular amyloid-β in TgCRND8 mice.
      McLean, Daniel, et al.
      ACS Chem Neurosci, 4: 613-23 (2013)  2013

      Show Abstract
      23509918 23509918
      Prion-like behaviour and tau-dependent cytotoxicity of pyroglutamylated amyloid-β.
      Nussbaum, Justin M, et al.
      Nature, 485: 651-5 (2012)  2012

      Show Abstract
      22660329 22660329