MABC962 Anti-ATG14 Antibody, clone 14G11.1

MABC962
100 μg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABC962
      DescriptionAnti-ATG14 Antibody, clone 14G11.1
      Alternate Names
      • Beclin 1-associated autophagy-related key regulator
      • ATG14 autophagy related 14 homolog
      • Atg14L
      • Autophagy-related protein 14-like protein
      • Barkor
      • Beclin 1-Interacting protein
      Background InformationBeclin 1-associated autophagy-related key regulator (UniProt Q6ZNE5; also known as ATG14 autophagy related 14 homolog, Atg14L, Autophagy-related protein 14-like protein, Barkor, Beclin 1-Interacting protein) is encoded by the ATG14 (also known as ATG14L, BARKOR, KIAA0831) gene (Gene ID 22863) in human. The adaptor protein ATG14/Atg14L/Barkor function as a key driver in orchestrating the formation of autophagosomes, an essential process of autophagy-mediated protein degradation. ATG14 complexes with the class III PI3K catalytic subunit Vps34, the regulatory proteins Beclin 1, and p150 for targeting to the isolation membrane to initiate autophagosomes formation. ATG14 is reported to be negatively regulated by G-protein-coupled receptor (GPCR) signaling, where GPCR-dependent activation of ZBTB16 leads to the degradation of Atg14L and autophagy inhibition.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-ATG14 Antibody, clone 14G11.1 is an antibody against ATG14 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant human ATG14 internal fragment.
      EpitopeInternal.
      Clone14G11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both spliced isoforms reported by UniProt (Q6ZNE5).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ATG14
      • ATG14L
      • BARKOR
      • KIAA0831
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~60 kDa observed. Target band size appears larger than the calculated molecular weights of 55.3 kDa (isoform 1) and 42.4 kDa (isoform 2).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SK-OV-3 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected ATG14 in 10 µg of SK-OV-3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications