ST1053 Anti-20S Proteasome Core Subunits Rabbit pAb

ST1053
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, Yeast Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      ST1053-100UL
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      Stocked 
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          Plastic ampoule 100 ul
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          Description
          OverviewRecognizes the ~25-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7) in human erythrocytes, HeLa cells and 3T3 cells.
          Catalogue NumberST1053
          Brand Family Calbiochem®
          Application Data
          Detection of human 20S proteasome core subunits by immunoblotting. Sample: Whole cell lysate from human erythrocytes. Primary antibody: Anti-20S Proteasome Core Subunits Rabbit pAb (Cat. No. ST1053) (1:1000). Detection: chemiluminescence.
          References
          Product Information
          FormLiquid
          FormulationSerum diluted in PBS.
          Positive controlHeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoblotting Gong, X., et al. 2007. Cancer Res. 67, 5380.
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Paraffin Sections
          Application NotesImmunoblotting (1:1000-1:10,000, see application references)
          Immunoprecipitation (see comments)
          Paraffin Sections (1:100)
          Application CommentsThis antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenhuman erythrocyte proteasomes
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Yeast
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Irritant
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-20S Proteasome Core Subunits Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-20S Proteasome Core Subunits Rabbit pAb Certificates of Analysis

          TitleLot Number
          ST1053
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-July-2007
          ApplicationImmunoblotting (1:1000-1:10,000, see application references)
          Immunoprecipitation (see comments)
          Paraffin Sections (1:100)
          Application Data
          Detection of human 20S proteasome core subunits by immunoblotting. Sample: Whole cell lysate from human erythrocytes. Primary antibody: Anti-20S Proteasome Core Subunits Rabbit pAb (Cat. No. ST1053) (1:1000). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes the ~27-30 kDa 20S proteasome core subunits (α5, α7, β1, β5i, and β7).
          BackgroundProteasomes are large multi-subunit complexes, that are localized in the nucleus and cytosol, and selectively degrade intracellular proteins. They play a major role in the degradation of many proteins involved in cellular processes such as cell cycling, apoptosis, and proliferation. The human proteasome contains two copies of each of seven different α-type and β-type subunits assembled to form a stack of four seven-membered rings. The α-subunits comprise the outer rings while the β-subunits comprise the inner rings. The β-subunits are believed to be responsible for the proteolytic activity of the 20S proteasome.
          HostRabbit
          Immunogen speciesHuman
          Immunogenhuman erythrocyte proteasomes
          IsotypeIgG
          Specieshuman, mouse, yeast
          Positive controlHeLa cells, RSV 3T3 mouse fibroblasts, or yeast whole cell lysates
          FormLiquid
          FormulationSerum diluted in PBS.
          Preservative≤0.1% sodium azide
          CommentsThis antibody has also been reported to work for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Irritant
          Application referencesImmunoblotting Gong, X., et al. 2007. Cancer Res. 67, 5380.