539166 Anti-19S Proteasome S2-Subunit Rabbit pAb

539166
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      539166-100UL
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          Glass bottle 100 ul
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          Description
          OverviewRecognizes the ~100 kDa 19S proteasome S2-subunit in HeLa cells and partially purified 26S proteasome preparations. Also recognizes an ~50 kDa potential subunit degradation product.
          Catalogue Number539166
          Brand Family Calbiochem®
          Application Data
          Detection of human 19S proteasome S2-subunit by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-19S Proteasome S2-Subunit Rabbit pAb (Cat. No. 539166) (1:1000). Detection: chemiluminescence.
          References
          ReferencesKloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol. 2, 179.
          Yew, P.R. 2001. J. Cell Physiol. 187, 1.
          Chaturvedi, K., et al. 2000. Biopolymers 55, 334.
          Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys. 383, 1.
          Pickart, C.M. 2000. Trends Biochem Sci. 25, 544.
          Product Information
          FormLiquid
          FormulationIn PBS, 1 mg/ml BSA.
          Positive controlHeLa cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesImmunoblotting (1:1000)
          Immunoprecipitation (see comments)
          Application CommentsAlso detects an ~50 kDa band, presumably corresponding to a subunit degradation product. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogenrecombinant, human 19S proteasome S2-subunit
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-19S Proteasome S2-Subunit Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-19S Proteasome S2-Subunit Rabbit pAb Certificates of Analysis

          TitleLot Number
          539166

          References

          Reference overview
          Kloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol. 2, 179.
          Yew, P.R. 2001. J. Cell Physiol. 187, 1.
          Chaturvedi, K., et al. 2000. Biopolymers 55, 334.
          Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys. 383, 1.
          Pickart, C.M. 2000. Trends Biochem Sci. 25, 544.

          Citations

          Title
        • Emily I. Chen, et al. (2005) Maspin alters the carcinoma proteome. FASEB Journal in press,.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-August-2007 RFH
          ApplicationImmunoblotting (1:1000)
          Immunoprecipitation (see comments)
          Application Data
          Detection of human 19S proteasome S2-subunit by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-19S Proteasome S2-Subunit Rabbit pAb (Cat. No. 539166) (1:1000). Detection: chemiluminescence.
          DescriptionPurified rabbit polyclonal antibody. Recognizes the ~100 kDa 19S proteasome S2-subunit protein.
          BackgroundProteolytic degradation is an important regulatory component of various cell processes including metabolism, stress response, antigen presentation, cell cycle regulation and modulation of cell surface receptors. The ubiquitin-proteasome pathway is the predominant mode of this proteolytic degradation. The 26S proteasome is an ATP-dependent multisubunit (~31 subunits), barrel shaped molecular machine with an apparent molecular weight of ~2.5 Mda. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. A series of enzymes is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Defects in the ubiquitin-proteasome pathway are associated with several genetic diseases including cystic fibrosis and Angelman's syndrome.
          HostRabbit
          Immunogen speciesHuman
          Immunogenrecombinant, human 19S proteasome S2-subunit
          IsotypeIgG
          Specieshuman
          Positive controlHeLa cells
          FormLiquid
          FormulationIn PBS, 1 mg/ml BSA.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsAlso detects an ~50 kDa band, presumably corresponding to a subunit degradation product. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesKloetzel, P.M. 2001. Nat. Rev. Mol. Cell Biol. 2, 179.
          Yew, P.R. 2001. J. Cell Physiol. 187, 1.
          Chaturvedi, K., et al. 2000. Biopolymers 55, 334.
          Orlowski, M., and Wilk, S. 2000. Arch. Biochem. Biophys. 383, 1.
          Pickart, C.M. 2000. Trends Biochem Sci. 25, 544.
          Citation
        • Emily I. Chen, et al. (2005) Maspin alters the carcinoma proteome. FASEB Journal in press,.