176908 Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis - Calbiochem

176908
  
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      Overview

      Replacement Information

      Key Specifications Table

      Description
      OverviewRecombinant, Bacillus anthracis anthrax protective antigen (nicked) expressed in a specialized strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
      Catalogue Number176908
      Brand Family Calbiochem®
      SynonymsPA 63
      References
      ReferencesMogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
      Nassi, S., et al. 2002. Biochemistry 41, 1445.
      Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
      Leppla, S.H. 1988. Methods Enzymol. 165, 103.
      Product Information
      ATP CompetitiveN
      FormLyophilized
      FormulationLyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.
      ReversibleN
      Applications
      Biological Information
      Primary TargetEF and/or LF
      PuritySingle major band at 63 kD by SDS-PAGE
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis - Calbiochem Certificates of Analysis

      TitleLot Number
      176908

      References

      Reference overview
      Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
      Nassi, S., et al. 2002. Biochemistry 41, 1445.
      Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
      Leppla, S.H. 1988. Methods Enzymol. 165, 103.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-September-2009 RFH
      SynonymsPA 63
      DescriptionRecombinant, Bacillus anthracis anthrax protective antigen(nicked) expressed in a special strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
      FormLyophilized
      FormulationLyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.
      PuritySingle major band at 63 kD by SDS-PAGE
      SolubilityReconstitute in sterile 50% glycerol or sterile, distilled H₂O to a final concentration of 1 mg/ml. Addition of BSA to a concentration of 1 mg/ml may enhance stability.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
      Toxicity Standard Handling
      ReferencesMogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
      Nassi, S., et al. 2002. Biochemistry 41, 1445.
      Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
      Leppla, S.H. 1988. Methods Enzymol. 165, 103.