176908 Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis - Calbiochem

176908
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      176908-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecombinant, Bacillus anthracis anthrax protective antigen (nicked) expressed in a specialized strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
          Catalogue Number176908
          Brand Family Calbiochem®
          SynonymsPA 63
          References
          ReferencesMogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
          Nassi, S., et al. 2002. Biochemistry 41, 1445.
          Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
          Leppla, S.H. 1988. Methods Enzymol. 165, 103.
          Product Information
          ATP CompetitiveN
          FormLyophilized
          FormulationLyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.
          ReversibleN
          Applications
          Biological Information
          Primary TargetEF and/or LF
          PuritySingle major band at 63 kD by SDS-PAGE
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          176908

          References

          Reference overview
          Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
          Nassi, S., et al. 2002. Biochemistry 41, 1445.
          Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
          Leppla, S.H. 1988. Methods Enzymol. 165, 103.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-September-2009 RFH
          SynonymsPA 63
          DescriptionRecombinant, Bacillus anthracis anthrax protective antigen(nicked) expressed in a special strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.
          FormLyophilized
          FormulationLyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.
          PuritySingle major band at 63 kD by SDS-PAGE
          SolubilityReconstitute in sterile 50% glycerol or sterile, distilled H₂O to a final concentration of 1 mg/ml. Addition of BSA to a concentration of 1 mg/ml may enhance stability.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
          Toxicity Standard Handling
          ReferencesMogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 7045.
          Nassi, S., et al. 2002. Biochemistry 41, 1445.
          Singh, Y., et al. 1999. Infect. Immun. 67, 1853.
          Leppla, S.H. 1988. Methods Enzymol. 165, 103.