176871 Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells

176871
  
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      Overview

      Replacement Information

      Key Specifications Table

      Description
      OverviewRecombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. Inactivates bradykinin by the sequential removal of two C-terminal dipeptides. ACE is a type I membrane protein that exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular mass of the glycosylated protein appears larger than 120 kDa under either reducing or non-reducing condition.
      Catalogue Number176871
      Brand Family Calbiochem®
      SynonymsPeptidyl-Dipetidase A, ACE
      References
      ReferencesWakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
      Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
      Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
      Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
      FormLiquid
      FormulationIn 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥1 unit/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsEndotoxins: ≤1.0 EU/µg ACE
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells SDS

      Title

      Safety Data Sheet (SDS) 

      Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells Certificates of Analysis

      TitleLot Number
      176871

      References

      Reference overview
      Wakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
      Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
      Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
      Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-April-2008 RFH
      SynonymsPeptidyl-Dipetidase A, ACE
      DescriptionRecombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. ACE gene polymorphism has been a recent topic in Alzheimer's disease studies. ACE is a type I membrane protein and exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular weight of the glycosylated protein appears larger to be than 120 kDa under both reducing and non-reducing conditions.
      FormLiquid
      FormulationIn 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxins: ≤1.0 EU/µg ACE
      Specific activity≥1 unit/µg protein
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesWakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
      Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
      Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
      Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.