SCR150 AldeRed™ ALDH Detection Assay

SCR150
1 Kit  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCR150
      DescriptionAldeRed™ ALDH Detection Assay
      Background InformationThe AldeRed™ ALDH Detection Kit provides cancer and stem cell scientists with new capabilities for live cell isolation and characterization. The AldeRed™ reagent is a red-shifted fluorescent substrate for aldehyde dehydrogenase (ALDH), allowing cells to be identified and isolated by flow cytometry with concurrent use of green fluorescent cell lines, antibodies, transgenic animals and reporter assays.

      Learn more about the AldeRed™ in Nature Communications! (Learn Now!)

      High ALDH activity serves as a universal marker of stem cells, both normal and malignant. Cells can be identified and isolated based upon the enzymatic activity of ALDH, a detoxifying enzyme responsible for oxidation of hazardous aldehyde byproducts. The marker ALDH has been used to isolate cancer stem cells from various human malignancies including bladder, breast, cervical, colon, head and neck, liver, lung, pancreas, prostate and ovary. The AldeRed™ assay employs a fluorescent and non-toxic ALDH substrate (AldeRed™ 588-A) that diffuses freely into intact and viable cells, but remains trapped inside the cells once converted by ALDH into the corresponding acid. The amount of fluorescence produced is proportional to the ALDH activity in the cells and is measured by flow cytometry, allowing fluorescence-activated cell sorting (FACS). This kit supplies the ALDH inhibitor diethylaminobenzaldehyde (DEAB), which is used in negative control testing necessary for background fluorescence assessement.
      References
      Product Information
      Components
      • 1. AldeRed™ Reagent: (Part No. CS216596). One (1) vial containing 50 µg of AldeRed™ reagent powder.
      • 2. DEAB Reagent: (Part No. CS216595). One (1) vial containing 1 mL of DEAB (Diethylaminobenzaldehyde) reagent.
      • 3. Hydrochloric Acid (2N): (Part No. CS216594). One (1) vial containing 1.5 mL of 2 Normal hydrochloric acid.
      • 4. Dimethylsulphoxide: (Part No. CS216593). One (1) vial containing 1.5 mL of Dimethylsulphoxide (DMSO).
      • 5. AldeRed™ Assay Buffer: (Part No. CS216592). Four (4) bottles containing 25 mL of AldeRed™ Assay Buffer each.
      • 6. Verapamil: (Part No. CS220012). Four (4) vials containing 615 µg Verapamil powder each.
      Detection methodFluorescence
      Applications
      ApplicationThe AldeRed™ system enables live cancer stem cell identification and isolation based on aldehyde dehydrogenase (ALDH) expression using a red-shifted fluorescent substrate for ALDH, allowing concurrent use of green fluorescent reporters including GFP cell lines.
      Key Applications
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting (FACS)
      Application Notes• For optimal excitation and emission of AldeRed™ 588-A, use a combination of blue laser (488 nm) or green laser (532 nm) with PE-Texas Red detector (615 nm). Detection in proximal measurement channels (e.g. PE) is also possible, but may affect delta MFI (mean fluorescence intensity) between Test sample and DEAB-treated control sample.
      • All live intact cells (even with low ALDH expression) become fluorescent upon exposure to activated AldeRed™ 588-A. The generation of a DEAB-treated control is necessary for each cell sample to establish baseline fluorescence.
      • Handle all reagents using aseptic techniques.
      Biological Information
      Physicochemical Information
      Emission max.
      • 615 nm
      Excitation max.
      • 488 nm
      • 532 nm
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each lot is tested by measuring ALDH activity in SK-BR-3 cells by flow cytometry.
      • AldeRed™ Reagent is 95% pure (PMR)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions• Store kit at 2-8°C.
      • Reconstituted and activated AldeRed™ Reagent may be stored in aliquots at -20°C protected from light for up to 1 year. Repeated freezing and thawing is not recommended.
      • AldeRed™ Assay Buffer supplemented with Verapamil may be stored at 2-8°C in aliquots for up to 3 months.
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      AldeRed™ ALDH Detection Assay SDS

      Title

      Safety Data Sheet (SDS) 

      AldeRed™ ALDH Detection Assay Certificates of Analysis

      TitleLot Number
      AldeRed ALDH Detection AssaySCR150
      AldeRed ALDH Detection Assay - 30605673060567
      AldeRed ALDH Detection Assay - 31278323127832
      AldeRed ALDH Detection Assay - 31763133176313
      AldeRed ALDH Detection Assay - 32195213219521
      AldeRed ALDH Detection Assay - 32458253245825
      AldeRed ALDH Detection Assay -27175612717561
      AldeRed ALDH Detection Assay -27271162727116
      AldeRed ALDH Detection Assay -28253372825337
      AldeRed ALDH Detection Assay Stem Cell Assay2956910

      References

      Reference overviewPub Med ID
      Nutrient deprivation in neuroblastoma cells alters 4-hydroxynonenal-induced stress response.
      Zimmermann, L; Moldzio, R; Vazdar, K; Krewenka, C; Pohl, EE
      Oncotarget  8173-8188  2017

      Show Abstract
      28030790 28030790
      Targeting cancer stem cell propagation with palbociclib, a CDK4/6 inhibitor: Telomerase drives tumor cell heterogeneity.
      Bonuccelli, G; Peiris-Pages, M; Ozsvari, B; Martinez-Outschoorn, UE; Sotgia, F; Lisanti, MP
      Oncotarget  9868-9884  2017

      Show Abstract
      28039467 28039467
      Chemosensitization and inhibition of pancreatic cancer stem cell proliferation by overexpression of microRNA-205.
      Chaudhary, AK; Mondal, G; Kumar, V; Kattel, K; Mahato, RI
      Cancer Lett  1-8  2017

      Show Abstract
      28536008 28536008
      Hybrid clone cells derived from human breast epithelial cells and human breast cancer cells exhibit properties of cancer stem/initiating cells.
      Gauck, D; Keil, S; Niggemann, B; Zänker, KS; Dittmar, T
      BMC Cancer  515  2017

      Show Abstract
      28768501 28768501