SCR022 Adult Rat Hippocampal Neural Stem Cells

SCR022
1 vial  1x10(e6) cells
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      Overview

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      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCR022
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAdult Rat Hippocampal Neural Stem Cells
      OverviewAdult Rat Hippocampal Neural Stem Cells

      Introduction:

      Neural stem cells (NSC) are self-renewing cells with the capacity to differentiate into neurons, astrocytes and oligodendrocytes. Adult NSC are extremely rare and are believed to reside only in discrete regions of the hippocampus and the subventricular zone. Due to their therapeutic promise, considerable attention has been focused on identifying the sources of stem cells, the signals that regulate their proliferation and the specification of neural stem cells towards more differentiated cell lineages (Gage, 2000).

      Presently, neural stem cells are often identified based upon the presence of molecular markers that are correlated with the stem and/or progenitor state along with the absence of differentiated phenotypes as assessed through marker analysis. To aid researchers in the accurate identification and characterization of neural stem cells, Millipore presents cryopreserved Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCR022).

      Millipore's Cryopreserved Adult Rat Hippocampal Neural Stem Cells provides ready-to use primary neural stem cells isolated from the hippocampus of adult Fisher 344 rats. These primary cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.

      We recommend that Millipore's Cryopreserved Adult Rat Hippocampal Neural Stem Cells (Catalog No. SCR022) be used in conjunction with the Neural Stem Cell Marker Characterization Kit (Catalog No. SCR019) and differentiation assays that demonstrate multipotentiality of the starting cell population. Please look for these differentiation assays from CHEMICON® in the near future.

      For Research Use Only; not for use in diagnostic procedure.
      Materials Required but Not Delivered1. Rat Neural Stem Cell Expansion Medium (e.g. DMEM/F12, B27 supplement, 2 mM L-glutamine and 1X solution of penicillin, streptomycin and fungizone (PSF))

      2. Basic fibroblast growth factor (bFGF; FGF-2, Millipore Catalog No. GF003)

      3. Poly-L-ornithine (Sigma Catalog No. P3655)

      4. Laminin (Millipore Catalog No. CC095)

      5. Accutase™ (Millipore Catalog No. SCR005)

      6. Tissue culture-ware

      7. Phosphate-Buffered Saline (1X PBS) (Millipore Catalog No. BSS-1005-B)

      8. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)

      9. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)

      10. Primary and secondary antibodies

      11. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution

      12. Anti-fading mounting solution (DABCO/PVA)

      13. Hemacytometer

      14. Microscope
      References
      Product Information
      Components
      • 1.1x10e6 viable Adult Rat Hippocampal Neural Stem Cells: (Part No. 2003769) derived from adult female Fisher 344 rats, aged 43-55 days, cryopreserved. Store in liquid nitrogen.
      Applications
      ApplicationAdult Rat Hippocampal Neural Stem Cells are self-renewing cells with the capacity to differentiate into neurons, astrocytes & oligodendrocytes.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Neural Stem Cells
      Cell Line Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at the recommended storage conditions (liquid nitrogen), adult hippocampal neural stem cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.
      Packaging Information
      Material Size1 vial
      Material Package1x10(e6) cells
      Transport Information
      Supplemental Information
      Specifications