178375 | APO-10 - CAS 2190-95-6 - Calbiochem

178375
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₁₂H₂₇PO 2190-95-6

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      178375-1GM
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          Plastic ampoule 1 gm
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          Description
          OverviewNovel non-ionic detergent useful for plasmid DNA isolation. Plasmid DNA isolated using APO-10 does not require the use of phenol/chloroform or proprietary resins. Provides excellent yields of high quality DNA. Allows plasmid DNA to be sequenced through difficult regions without the need to subclone into single-stranded vectors.
          Catalogue Number178375
          Brand Family Calbiochem®
          SynonymsDimethyldecylphosphine Oxide
          References
          ReferencesLi, L., et al. 2003. Hypertension 42, 997.
          Altschuler, M., et al. 1994. Biotechniques 17, 434.
          Product Information
          CAS number2190-95-6
          Aggregation Number131
          CMC4.6 mM
          FormWhite solid
          Hill FormulaC₁₂H₂₇PO
          Chemical formulaC₁₂H₂₇PO
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at room temperature.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          178375

          References

          Reference overview
          Li, L., et al. 2003. Hypertension 42, 997.
          Altschuler, M., et al. 1994. Biotechniques 17, 434.

          Brochure

          Title
          Detergents and Buffers Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-April-2008 RFH
          SynonymsDimethyldecylphosphine Oxide
          DescriptionNovel non-ionic detergent useful for plasmid DNA isolation (aggregation number: 131; CMC: 4.6 mM). Plasmid DNA isolated using APO-10 does not require the use of phenol/chloroform or proprietary resins. Provides excellent yields of high quality DNA. Allows plasmid DNA to be sequenced through difficult regions without the need to subclone into single-stranded vectors.
          FormWhite solid
          Recommended reaction conditions
          Protocol for Plasmid DNA Isolation using APO-10 1. Centrifuge 1.5 ml of bacterial culture at 12,000 x g for 2 min. 2. Resuspend the pellet in 100 µl of 50 mM glucose, 25 mM Tris-HCl, 10 mM EDTA, 10 µg/ml RNase, pH 8.0. 3. Add 200 µl of 200 mM NaOH and 1% APO-10. Mix gently by inverting. Incubate at room temperature for 5 min. 4. Add 150 µl of 3 M potassium acetate buffer, pH 4.8. Mix gently by inverting. Incubate at room temperature for 5 min. 5. Centrifuge sample for 15 min at 4°C. Collect the supernatant. 6. Add 1 ml of 100% ethanol to the supernatant, mix, and centrifuge for 15 min at 4°C. Wash the pellet in 70% ethanol and dry. 7. The DNA pellet can be resuspended in 25 µl TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA). RNase A can be added to digest any RNA.
          CAS number2190-95-6
          Chemical formulaC₁₂H₂₇PO
          Structure formulaStructure formula
          SolubilityH₂O (10 mg/ml)
          Storage +15°C to +30°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at room temperature.
          Toxicity Irritant
          ReferencesLi, L., et al. 2003. Hypertension 42, 997.
          Altschuler, M., et al. 1994. Biotechniques 17, 434.