194437 | ABTS™ Chromophore, Diammonium Salt

194437
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      194437-1EA
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          Description
          OverviewContains 20 tablets. Each tablet contains 5 mg and makes 5 ml of ABTS™ Chromophore working substrate for ELISA.
          Catalogue Number194437
          Brand Family Calbiochem®
          References
          Product Information
          CAS number30931-67-0
          FormSolid
          Hill FormulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
          Chemical formulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSDL7002000
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsUnstable in solution; reconstitute just prior to use and discard any unused reconstituted substrate.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          194437

          Brochure

          Title
          Enzyme-Linked Detection Systems Technical Bulletin
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision06-March-2009 JSW
          DescriptionUseful as a substrate for ELISA; acts as a hydrogen donor in peroxidase reactions. Radical cation has an absorption maximum at 414 nm.
          FormSolid
          Recommended reaction conditions
          For Use as ELISA Substrate Note: Each tablet contains 5 mg and yields 5 ml ABTS® working substrate for ELISA when reconstituted as outlined below. 1. Dissolve ABTS at a final conc. of 1 mg/ml in 0.1 M sodium citrate buffer at pH 4.2. 2. Add 1.8 µl 30% H2O2 to 6 ml of the 1 mg/ml ABTS solution above (or 3 µl 3% H2O2 to 1 ml of the ABTS solution). 3. Add 1 ng HRP-conjugated antibody or HRP to 1 ml of the ABTS solution containing H2O2. 4. Incubate for 30 min to 1 h at 37°C (room temp is also OK, but the reaction is faster at 37°C). 5. Stop the reaction at the end of incubation with 1 ml (i.e. equal volume) of stop solution (0.625 M oxalic acid) and read the absorbance at 405 nm.
          CAS number30931-67-0
          RTECSDL7002000
          Chemical formulaC₁₈H₁₈N₄O₆S₄ · (NH₃)₂
          Solubility0.1 M sodium citrate buffer, pH 4.2 (1 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsUnstable in solution; reconstitute just prior to use and discard any unused reconstituted substrate.
          Toxicity Irritant