480706 | α2,3-Neuraminidase, Macrobdella decora, Recombinant, E. coli

480706
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      480706-50MIU
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          Glass bottle 50 miu
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          Description
          OverviewRecombinant, Macrobdella decora α2,3-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of α2,3-linked N-acetylneuraminic acid from complex oligosaccharides. Will catalyze the cleavage of α2,3-linkages from α1-acid glycoprotein, fetuin, keratan sulfate, sialyl Lewis x, and 3′-sialyllactose, yielding 2,7-anhydro-Neu5Ac instead of “free Neu5Ac” as the hydrolysis product.
          Note: 1 mU = 1 milliunit.
          Catalogue Number480706
          Brand Family Calbiochem®
          SynonymsSialidase L
          References
          ReferencesChou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.
          Product Information
          CAS number9001-67-6
          Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 nmol methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.5.
          EC number3.2.1.18
          FormLiquid
          FormulationIn 10 mM phosphate buffer, 2% glycerol, pH 7.0.
          Applications
          Biological Information
          Specific Activity≥2000 U/mg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          ContaminantsCeramide glycanase, α- and β-galactosidase, β-hexosaminidase, α- and β-mannosidase, proteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSQQ3450000
          Safety Information
          R PhraseR: 20-36/37/38-42/43

          Harmful by inhalation.
          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation and skin contact.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          480706

          References

          Reference overview
          Chou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision01-June-2009 JSW
          SynonymsSialidase L
          DescriptionRecombinant, Macrobdella decora α2,3-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of α2,3-linked N-acetylneuraminic acid from complex oligosaccharides. Will catalyze the cleavage of α2,3-linkages from α1-acid glycoprotein, fetuin, keratan sulfate, sialyl Lewis x, and 3'-sialyllactose, yielding 2,7-anhydro-Neu5Ac instead of "free Neu5Ac" as the hydrolysis product.
          FormLiquid
          FormulationIn 10 mM phosphate buffer, 2% glycerol, pH 7.0.
          Concentration Label Please refer to vial label for lot-specific concentration
          CAS number9001-67-6
          RTECSQQ3450000
          EC number3.2.1.18
          ContaminantsCeramide glycanase, α- and β-galactosidase, β-hexosaminidase, α- and β-mannosidase, proteases: none detected
          Specific activity≥2000 U/mg protein
          Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1 nmol methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.5.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Toxicity Harmful
          ReferencesChou, M.-Y., et al. 1996. J. Biol. Chem. 271, 19219.