344826 | α1,6-Fucosidase, Chryseobacterium meningosepticum, Recombinant, E. coli

344826
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      344826-50MIU
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          Plastic ampoule 50 miu
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          Description
          OverviewRecombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium) meningosepticum catalyzes the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-Linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
          Note: 1 mU = 1 milliunit.
          Catalogue Number344826
          Brand Family Calbiochem®
          Synonymsα-L-Fucoside Fucohydrolase
          References
          ReferencesZheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.
          Product Information
          CAS number9037-65-4
          Activity≥1.0 unit/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.
          EC number3.2.1.51
          FormLiquid
          FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
          Applications
          Biological Information
          Specific Activity≥1.5 units/mg protein
          Physicochemical Information
          ContaminantsProteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          344826

          References

          Reference overview
          Zheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-October-2007 JSW
          Synonymsα-L-Fucoside Fucohydrolase
          DescriptionRecombinant, Chryseobacterium meningosepticum α1,6-fucosidase expressed in E. coli. This enzyme from Chryseobacterium (formerly Flavobacterium meningosepticum) catalyses the hydrolysis of branched non-reducing terminal α1,6-linked fucose residues from the N-acetylglucosamine core of N-linked oligosaccharides. The reducing terminus of the oligosaccharide must be covalently bonded to a reporter molecule, such as aminonaphthalene disulfonic acid (ANDSA), aminonaphthalene trisulfonate (ANTS), and 2-aminoanthanilic acid (2-AA). The one exception is that a terminal unbranched α1,3-fucose is cleaved in the absence of any reporter molecule. Does not cleave fucose from glycoproteins.
          FormLiquid
          FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
          Recommended reaction conditions
          50 mM sodium phosphate buffer, pH 5.0.
          CAS number9037-65-4
          EC number3.2.1.51
          ContaminantsProteases: none detected
          Specific activity≥1.5 units/mg protein
          Activity≥1.0 unit/ml
          Unit definitionOne unit is defined as the amount of enzyme that will release 1.0 µmol methylumbelliferone from 4-methylumbelliferyl-α-L-fucoside per min at 37°C, pH 5.0.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesZheng, Q., et al. 2002. J. Biol. Chem. 277, 39823.