Flow Cytometry Kits for Cell Health
Featured Cell Health Product
|FlowCellect™ MitoDamage Kit
Analyzed Dual Parameter Data: Dot plots depicting Jurkat cells treated with multiple inducers and stained using MitoDamage Kit. Jurkat cells were treated with 2 µM Staurosporine or 50 µM CCCP and then stained using the MitoDamage Kit. Plots show the percentage of positive cells for 1) Apoptosis (Annexin V binding) and mitochondrial membrane potential change (Red2), 2) Cell death and mitochondrial membrane potential change, and 3) apoptosis and cell death.
|guava ViaCount® Reagent||100 Tests||4000-0040|
|guava ViaCount® Reagent||600 Tests||4000-0041|
|guava ViaCount® Flex Reagent Kit||100 Tests||4500-0110|
|guava ViaCount® Flex Reagent Kit||500 Tests||4700-0060|
|guava ViaCount® Cell Dispersal Reagent for Flow Cytometry||100 Tests||4700-0050|
A variety of FlowCellect™ Kits can now be employed to assess changes in mitochondrial membrane potential, apoptosis as measured by Annexin V binding, mitochondrial oxidative stress, and cell death.
|FlowCellect™ MitoPotential Red Kit|
|FlowCellect™ MitoDamage Kit|
|FlowCellect™ MitoStress Kit|
|FlowCellect™ Cytochrome C Kit|
|FlowCellect™ Oxidative Stress Characterization Kit|
|guava Mitochondrial Depolarization Assay Kit|
The Guava EasyCyte™ Cell Growth reagent uses a simple, non-radioactive protocol for measurement of cell growth by a combination of carboxyfluorescein diacetate succinimidyl ester (CFSE) and Propidium Iodide (PI). The kit can be used to measure growth out to five generations.
Applications for EasyCyte™ Cell Growth Reagent:
|Anti-MAD2A-FITC, clone 17D10|
|Anti-Y14-FITC, clone 4C4|
|Anti-FXR1-FITC, clone 6BG10|
|Anti-SMN-FITC, clone 2B1|
|Anti-CAF1 p150-FITC, clone SS1, 1-3|
|Anti-RPA2 p34 -FITC, clone RPA20 1-46|
|Anti-RPA1 p70 -FITC, clone RPA9, 1-30|
|Anti-BMI1-FITC, clone AF27|
|Anti-RBMS1-FITC, clone 4D11|
|Anti-c-Jun-FITC, clone 6E4|
|Anti-RBMS1-FITC, clone 4D11|
|Phospho-ATM (Ser1981) - PE|
|Phospho H3 (Ser10)- Alexa 488|
|Phospho-SMC1 (Ser957) - Alexa 488|
|Anti-Cyclin B1 - PE Human|
MilliporeSigma’s FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kits provide a quantitative solution for studying autophagy and measuring potency of autophagy inducers using flow cytometry.
Two host cell lines are offered in either CHO (Chinese Hamster Ovary) or U20S (human osteosarcoma) to assist in your autophagy research. The CHO reporter cell line is ideal for flow cytometry applications, while U20S is suitable for both imaging and flow cytometry.
The following products are now available for use to evaluate autophagy:
|FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kit (CHO)|
|FlowCellect™ RFP-LC3 Reporter Autophagy Assay Kit|
|FlowCellect™ Autophagy LC3 Antibody-based Assay Kit|
|FlowCellect™ Autophagy Detection Reagent Pack|
When performed on the guava easyCyte™ system, apoptosis kits can determine the count of a cell population without using external bead sets. Multiparametric evaluation of apoptotic status is of great utility in studying impact of compounds on in drug screening studies. These kits provides a complete means of apoptotic status and its response for inducer treatment conditions or diseases.
|Early Apoptosis Kits|
|FlowCellect™ Annexin Red Kit (Annexin V-CF647 Reagent/7-AAD)|
|guava Nexin Reagent (Annexin V-PE/7AAD)|
|guava Nexin Reagent (500 test)|
|Mid Apoptosis Kits|
|guava Caspase 8 FAM Kit|
|guava MultiCaspase SR and Caspase 3/7 FAM Kit|
|guava MultiCaspase SR Kit|
|guava MultiCaspase FAM Kit|
|guava Caspase 3/7 FAM Kit|
|Late Aptoptosis Kits|
|guava TUNEL Reagent Kit (Anti-BrdU-TRITC/ TdT Enzyme/ Br-dUTP)|
|Apoptosis Signaling Kit|
|FlowCellect™ Bcl-2 Activation Dual Detection Kit (Anti-Bcl-2-Alexa Fluor 488/Anti-pBcl-2 (Ser70)-PE)|
Cell cycle phase distributions can be used to assess cell health, proliferation, as well as the potential mechanism of antineoplastic agents. For example, measuring the population of S phase cells can reflect the amount of newly synthesized DNA. Also, distinguishing cells in G2 from M phase cells can help identify cells undergoing mitosis.
Guava and FlowCellect™ Kits for Cell Cycle provide many advantages for evaluation of cell cycle.
|FlowCellect™ Bivariate Cell Cycle Kit for DNA Replication Analysis|
|FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis|
|guava Cell Cycle Reagent|