Cellular processes such as drug uptake and processing by cells, entry of pathogens and toxins, or antigen processing and presentation are studied on Amnis® imaging flow cytometers by measuring internalization. Amnis® imaging flow cytometry systems automatically collect large numbers of images per data set and provides quantitative image analysis tools that facilitate the objective and rapid localization of internalized probes. Because the ImageStream®X collects multiple fluorescent images per cell, internalized markers in phenotypically distinct sub-populations as well as co-localization to different cellular compartments (see Co-localization) can be simultaneously determined in a quantitative manner.
Watch to learn how multispectral imaging in flow can be used to answer specific internalization research questions. Dr. Sherree Friend explains how Amnis® applications use high-throughput imaging to observe primary dendritic cells to provide insight regarding cellular internalization of antigen and other particles.