The Amnis® imaging flow cytometry system is advancing the study of cell death and survival. Morphological characterization by microscopy remains the gold standard for accurately identifying the various types of cell death using characteristics such as nuclear condensation, nuclear fragmentation, membrane blebbing, cell shrinkage or swelling. In some cases cell death is preceded by an attempt at survival by autophagy and is identified by the clustering of the phagolysosome membrane-associated protein LC3. Combining the measurements of cell death with immunophenotyping or other Amnis® imaging flow cytometry applications such as signal transduction increases the power of your experiments.
Watch to learn how multispectral imaging in flow can be used to enhance apoptosis research. Dr. Sherree Friend explains how Amnis® applications use high-throughput imaging of DNA fragmentation in apoptotic cells. Assessment of the process of cell death is revolutionized by analyzing visual characteristics in thousands of cells.