FCMAB171C5 | Milli-Mark™ Anti-CD4 -PECy5 Antibody, clone MT310

Flow Cytometry Analysis:
Representative lot data.
FACS analysis of anti-human CD4-PE/Cy5 stained human lymphocytes. Cells expressing CD4 (right). CD marker expression levels can vary between different blood specimens.
Flow Cytometry Analysis:
Representative lot data.
FACS analysis of anti-human CD4-PE/Cy5
100 tests  
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      Key Spec Table

      Species Reactivity Key Applications Host Format Antibody Type
      HFCMPhycoerythrin/Cy5Monoclonal Antibody
      Catalogue Number FCMAB171C5
      Synonyms T-cell surface antigen T4/Leu-3
      Trade Name
      • Milli-Mark
      Description Milli-Mark™ Anti-CD4 -PECy5 Antibody, clone MT310
      Background Information CD4 is a 55 kDa glycoprotein with four immunoglobulin-like external domains, a transmembrane domain and a highly conserved intracellular domain (Leong, 1999). CD4 acts as a coreceptor in MHC class II-restricted antigen-induced T-cell activation. Further, CD4 has a role in the regulation of T-B lymphocyte adhesion in the absence of antigen recognition, and in thymic differentiation (Piatier-Tonneau, 2002). CD4 is expressed on thymocyte subsets, it marks helper/inducer T cells, and it is expressed in 55-65% of mature peripheral T cells. Subpopulations of suppressor or cytotoxic T cells can be identified among CD4-positive T cells. CD4 is also expressed on monocytes/macrophages, Langerhans cells and other dendritic cells. CD4 is not expressed on B cells. Anti-CD4, MT310, was included in the Second International Workshop and Conference on Human Leucocyte Differentiation Antigens, and studies by a number of laboratories confirmed its reactivity with the CD4 antigen (Miedema, 1986).
      Product Information
      Format Phycoerythrin/Cy5
      Specificity Antibody recognizes Human CD4 antigen.
      • Human Lymphocytes
      Presentation Purified Mouse monoclonal IgG1 kappa conjugated to PE-Cy5 in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
      Application Detect CD4 using this Milli-Mark Anti-CD4 -PECy5 Antibody, clone MT310 validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      Clone MT310
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Antibody recognizes Human CD4 antigen.
      Isotype IgG1κ
      Species Reactivity Human
      Species Reactivity Note Tested and passed on Human.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Purification Method Protein A purfied
      UniProt Number
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by flow cytometry using human lymphocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size 100 tests
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies