MAB4401 | Anti-Oct-4 Antibody, clone 10H11.2

A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).
A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast
MAB4401
100 µg  
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      Overview

      Offerte speciali

      Tabella delle specifiche principali

      Species Reactivity Key Applications Host Format Antibody Type
      H ELISA,WB,FC,ICC M Purified Monoclonal Antibody
      Description
      Catalogue Number
      Brand Family Chemicon®
      Synonyms
      Trade Name
      • Chemicon
      Description Anti-Oct-4 Antibody, clone 10H11.2
      Overview Purified monoclonal antibody for the detection of Human Oct-4.
      Background Information Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.
      References
      Product Information
      Format Purified
      Specificity Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
      Control
      • Human embryonic stem cell lysate.
      Presentation Purified Ascites presented as a liquid in PBS with 0.1% sodium azide.
      Applications
      Application Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
      Key Applications
      • ELISA
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      Application Notes Flow Cytometry: A previous lot of this antibody was used in FC.

      ELISA: A previous lot of this antibody was used in ELISA.

      Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen Recombinant GST fusion protein, human Oct-4 (aa 1-134).
      Clone clone 10H11.2
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).
      Isotype
      Species Reactivity
      Species Reactivity Note The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Oct-4
      • POU5F1
      • Oct-3
      • MGC22487
      • Oct4
      • OTF4
      • OTF3
      • OCT3
      • OCT4
      Purification Method Protein A purfied
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
      SIZE: 360 amino acids; 38571 Da
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
      SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
      MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Evaluated by western blot on mouse P1 brain lysates.

      Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificati d'Analisi

      TitoloNumero di lotto
      Anti-Oct-4, clone 10H11.2 2474968
      Anti-Oct-4, clone 10H11.2 - 2392284 2392284
      Anti-Oct-4, clone 10H11.2 - 1991253 1991253
      Anti-Oct-4, clone 10H11.2 - 2066310 2066310
      Anti-Oct-4, clone 10H11.2 - LV1679970 LV1679970
      Anti-Oct-4, clone 10H11.2 - LV1786447 LV1786447
      Anti-Oct-4, clone 10H11.2 - NG1865606 NG1865606
      Anti-Oct-4, clone 10H11.2 - NG1927045 NG1927045
      Anti-Oct-4, clone 10H11.2 - PSO1553824 PSO1553824
      Anti-Oct-4, clone 10H11.2 -2512486 2512486

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      High-content imaging-based screening of microenvironment-induced changes to stem cells.
      Sebasti Vega,Er Liu,Parth J Patel,Anthony B Kulesa,Aaron L Carlson,Yanrui Ma,Matthew L Becker,Prabhas V Moghe
      Journal of biomolecular screening 17 2012

      Mostra il sommario
      22811477
      Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
      Chien-Hong Chen,Jie Xu,Wei-Fang Chang,Chia-Chia Liu,Hwa-Yun Su,Y Eugene Chen,Fuliang Du,Li-Ying Sung
      Reproductive biomedicine online 25 2012

      Mostra il sommario
      22877942
      Oct-4B isoform is differentially expressed in breast cancer cells: hypermethylation of regulatory elements of Oct-4A suggests an alternative promoter and transcriptional start site for Oct-4B transcription.
      Wang, Yajuan, et al.
      Biosci. Rep., 31: 109-15 (2011) 2011

      Mostra il sommario
      20433421
      Characterization of differential properties of rabbit tendon stem cells and tenocytes.
      Jianying Zhang,James H-C Wang
      BMC musculoskeletal disorders 11 2010

      Mostra il sommario Testo completo dell'articolo
      20082706
      Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes.
      Sung LY, Chang CC, Amano T, Lin CJ, Amano M, Treaster SB, Xu J, Chang WF, Nagy ZP, Yang X, Tian XC
      Cell Reprogram 12 203-11. 2010

      Mostra il sommario
      20677934
      Ago2 immunoprecipitation identifies predicted microRNAs in human embryonic stem cells and neural precursors.
      LA Goff, J Davila, MR Swerdel, JC Moore, RI Cohen, H Wu, YE Sun, RP Hart
      PloS one 4 e7192 2009

      Mostra il sommario Testo completo dell'articolo
      19784364
      The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
      Patricia A Zuk, Patricia A Zuk
      Journal of cellular biochemistry 106 867-77 2009

      Mostra il sommario
      19199344
      Microporous membrane growth substrates for embryonic stem cell culture and differentiation.
      Steven D Sheridan,Sonia Gil,Matthew Wilgo,Aldo Pitt
      Methods in cell biology 86 2008

      Mostra il sommario
      18442643
      Targeting cancer stem cells through L1CAM suppresses glioma growth.
      Bao, Shideng, et al.
      Cancer Res., 68: 6043-8 (2008) 2008

      Mostra il sommario
      18676824

      Brochure

      Titolo
      Human Stem Cell Systems

      Informazioni tecniche

      Titolo
      A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
      Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs

      Scheda tecnica

      Titolo
      Anti-Oct-4, clone 10H11.2 - Data Sheet
      STEMCCA Lentivirus Reprogramming Kits

      Prodotti e applicazioni correlate

      Alternative Packsize

       Numero di catalogo Descrizione  
      MAB4401-KC Anti-Oct-4, clone 10H11.2 (1mg) KC Prezzi e disponibilità

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      Categorie

      Life Science Research > Antibodies and Assays > Primary Antibodies