MAB4365 | GCTM-5 Antibody, clone GCTM-5

CFPAC1 cells fixed and labeled with 5 μg/mL of GCTM-5 antibody (green); cells are also labeled with DAPI (blue) to visualize all nuclei. A subset of the cells are labeled with GCTM-5, a normal pattern of staining for this antibody. The GCTM-5 antibody labeling was visualized with an Alexa 488-conjugated goat anti-mouse secondary antibody.
CFPAC1 cells fixed and labeled with 5 μg/mL of GCTM-5 antibody (green); cells are also labeled with
100 µg  
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      Tabella delle specifiche principali

      Species Reactivity Key Applications Host Format Antibody Type
      H ICC, WB, IHC M Purified Monoclonal Antibody
      Catalogue Number MAB4365
      Trade Name
      • Chemicon
      Description GCTM-5 Antibody, clone GCTM-5
      Background Information The GCTM-5 monoclonal antibody reacts with a minority sub-population of cells in spontaneously differentiating cultures of pluripotent human embryonic stem cells and embryonal carcinoma. The epitope recognized by GCTM-5 is found on a 50-kDa protein present on the surface of these cells. Previous studies suggest that GCTM-5 may also prove to be a useful tool for defining cell lineage relationships between putative progenitor populations in embryonic liver and biliary epithelium during tissue repair (Pera M, et al, 2005).
      Product Information
      Format Purified
      Presentation Purified mouse monoclonal IgG1 with 0.05% sodium azide.
      Application GCTM-5 Antibody, clone GCTM-5 is an antibody against GCTM-5 for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      Immunogen Membrane prepared from testicular seminoma
      Clone GCTM-5
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity This antibody reacts with GCTM-5 antigen that is expressed upon the cell surface of human embryonal carcinoma, differentiating cultures of human embryonic stem cells, and bilary epithelial cells.
      Isotype IgG1
      Species Reactivity Human
      Species Reactivity Note Reacts with human. Reactivity to other species has not yet been determined.
      Antibody Type Monoclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Maintain refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
      Packaging Information
      Material Size 100 µg
      Transport Information
      Supplemental Information




      Certificati d'Analisi

      TitoloNumero di lotto
      GCTM-5 Antibody, clone GCTM-5 - 21095462109546
      GCTM-5 Antibody, clone GCTM-5 - 22835492283549
      Monoclonal Antibody - LV1478901LV1478901

      Riferimenti bibliografici

      Panoramica dei riferimenti bibliograficiCodice d'identificazione nel Pub Med
      Transcriptional analysis of early lineage commitment in human embryonic stem cells.
      Laslett, Andrew L, et al.
      BMC Dev. Biol., 7: 12 (2007) 2007

      Mostra il sommario
      Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
      Adewumi O, et al.
      Nat. Biotechnol., 25: 803-16 (2007) 2007

      Liver stem cells--prospects for clinical use.
      Tosh, David and Strain, Alastair
      J. Hepatol., 42 Suppl: S75-84 (2005) 2005

      The hESC line Envy expresses high levels of GFP in all differentiated progeny.
      Costa, Magdaline, et al.
      Nat. Methods, 2: 259-60 (2005) 2005

      A novel cell-surface marker found on human embryonic hepatoblasts and a subpopulation of hepatic biliary epithelial cells.
      Stamp, Lincon, et al.
      Stem Cells, 23: 103-12 (2005) 2005



      Human Stem Cell Systems

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      Life Science Research > Antibodies and Assays > Primary Antibodies