SCR051 | Bone Marrow Harvesting and Hematopoietic Stem Cell Isolation Kit

SCR051
10 isolations/kit  
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      Principales applications
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      Description
      Référence SCR051
      Description Bone Marrow Harvesting and Hematopoietic Stem Cell Isolation Kit
      Aperçu The Bone Marrow Harvesting and Hematopoietic Stem Cell Isolation Kit provides an improved methodology based on sequential grinding and enzymatic digestion of murine bone tissue for harvesting higher numbers of BM cells and HSC from the endosteal and central marrow regions.
      Informations générales Hematopoietic stem cells (HSC) are frequently located in close proximity to bone lining cells within the endosteum. Recent studies demonstrated the significance of the endosteal region for hematopoiesis, and in particular that it contains a high number of HSCs. Consequently, traditional methods for harvesting bone marrow (BM) from mice via flushing of the long bones do not maximize the recovery of all HSCs. The Bone Marrow Harvesting and Hematopoietic Stem Cell Isolation Kit permits the optimal recovery of total cells and HSCs from mouse bones, and uniquely the isolation of HSCs specifically from the endosteal region. A combination of mechanical fragmentation by grinding of bones and enzymatic digestion is used for this purpose. Using this method, HSCs isolated specifically from the endosteum have been demonstrated to have a greater hematopoietic potential than HSCs isolated from the central marrow core including a 1.8-fold greater proliferative potential, an almost 2-fold greater ability to home to the bone marrow following tail vein injection and a significantly greater long-term hematopoietic reconstitution potential (Haylock, D. et al. Stem Cells, 2007). Additionally, this method has been shown to produce a 6-fold greater recovery of primitive HSCs (Lineage- Sca-1+ and c-Kit+) than traditional flushing methods.
      Références bibliographiques
      Informations produit
      Composants
      • 4. Collagenase I - Ten (10) single use vials of 30 mg powdered enzyme
      • Sufficient reagents are supplied in the kit for 10 separate isolations.
      • 1. Mortar and pestle - One (1) porcelain mortar and pestle
      • 2. Cell Strainer - Twenty (20) cell strainers with 40 µm nylon mesh and blue polypropylene frame
      • 3. Dispase II - Ten (10) single use vials of 40 mg powdered enzyme
      Applications
      Application The Bone Marrow Harvesting & Hematopoietic Stem Cell Isolation Kit permits the optimal recovery of total cells & HSCs from mouse bones & uniquely the isolation of HSCs specifically from the endosteal region.
      Principales applications
      • Cell Harvesting
      Informations biologiques
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Mortar, pestle, and cell strainers can be stored at room temperature.
      Single use vials containing the enzymes Dispase II and Collagenase I should be stored at 2° to 8°C until ready to use.
      Informations sur l'emballage
      Quantité 10 isolations/kit
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      FDS

      Langues
      English

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Granulocyte colony stimulating factor expands hematopoietic stem cells within the central but not endosteal bone marrow region.
      Jochen Grassinger,Brenda Williams,Gemma H Olsen,David N Haylock,Susan K Nilsson
      Cytokine 58 2012

      Afficher le résumé
      22341722
      Glucagon supports postabsorptive plasma glucose concentrations in humans with biologically optimal insulin levels.
      Cooperberg BA, Cryer PE
      Diabetes 59 2941-4. Epub 2010 Aug 10. 2010

      Afficher le résumé Article en texte intégral
      20699417
      Phenotypically identical hemopoietic stem cells isolated from different regions of bone marrow have different biologic potential.
      Jochen Grassinger,David N Haylock,Brenda Williams,Gemma H Olsen,Susan K Nilsson
      Blood 116 2010

      Afficher le résumé
      20631378
      Investigating the interactions between haemopoietic stem cells and their niche: methods for the analysis of stem cell homing and distribution within the marrow following transplantation.
      Brenda Williams,Susan K Nilsson
      Methods in molecular biology (Clifton, N.J.) 482 2009

      Afficher le résumé
      19089351

      Brochure

      Titre
      Cellutions Newsletter: 2007, Volume 1
      Cellutions Newsletter: 2008, Volume 1
      Cellutions Newsletter: 2008, Volume 2
      Cellutions Newsletter: 2008, Volume 3

      Informations techniques

      Titre
      Maximal Recovery of Hematopoietic Stem Cells from Bone Marrow Endosteum

      Manuels d'utilisation

      Titre
      Bone Marrow Harvesting & Hematopoietic Stem Cell Isolation Kit