MAB1637 | Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

Mouse anti-Beta III Tubulin (Catalog Number MAB1637). Localization of beta III Tubulin (red) and GFAP (green) in rat brain cortical cells thawed and cultured for 7 days. The cells were obtained from Combrex, Inc. Photo courtesy of QBMCell Science.
Mouse anti-Beta III Tubulin (Catalog Number MAB1637). Localization of beta III Tubulin (red) and GFA
MAB1637
100 µL  
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      Overview

      Replacement Information

      Offres spéciales

      Tableau de caractéristiques principal

      Species Reactivity Key Applications Host Format Antibody Type
      Av, Sh, R, Po, Mk, M, H, B ELISA, WB, IP, IH(P), IHC, ICC M Ascites Monoclonal Antibody
      Description
      Catalogue Number MAB1637
      Replaces 04-1049
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)
      Alternate Names
      • Tubulin beta-III
      • tubulin, beta 4
      • tubulin, beta 3
      Background Information Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.
      References
      Product Information
      Format Ascites
      Control
      • Positive control: Brain tissue or neurons of any type.
        Negative control: Non neural tissue or Astrocytes.
      Presentation Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
      Applications
      Application Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • Immunohistochemistry
      • Immunocytochemistry
      Application Notes Immunohistochemistry:
      On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.

      Immunoprecipitation:
      A previous lot of this antibody was used on Immunoprecipitation.

      Immunocytochemistry:
      1:100 to 1:500 dilution from a previous lot was used on fixed cells.

      Immunoblotting:
      1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.

      ELISA:
      A previous lot of this antibody was used on ELISA.

      Optimal working dilution must be determined by the end user.
      Biological Information
      Immunogen A synthetic peptide corresponding to amino acids 443-450 (ESESQGPK) of human class III beta-tubulin conjugated to KLH.
      Epitope C-terminus
      Clone TU-20 (Similar to TUJ1)
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Host Mouse
      Specificity The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.
      Isotype IgG1
      Species Reactivity
      • Avian
      • Sheep
      • Rat
      • Pig
      • Monkey
      • Mouse
      • Human
      • Bovine
      Species Reactivity Note Mouse. Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
      Antibody Type Monoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBB3
      • MC1R
      • beta-4
      • TUBB4
      Purification Method Unpurified
      UniProt Number
      UniProt Summary FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE: 450 amino acids; 50433 Da
      SUBUNIT: Dimer of alpha and beta chains.
      DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
      SIMILARITY: Belongs to the tubulin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Routinely evaluated by Western Blot on Mouse Brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size 100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificats d'analyse

      TitreNuméro de lot
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 23922412392241
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 24203602420360
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 20204822020482
      Anti-Tubulin, beta III isoform, -25499742549974
      Anti-Tubulin, beta III isoform, -25702242570224
      Anti-Tubulin, beta III isoform, -25834062583406
      Anti-Tubulin, beta III isoform, -26048182604818
      Anti-Tubulin, beta III isoform, -26367212636721
      Anti-Tubulin, beta III isoform, C-terminus, clone2462799
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 19707001970700
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 19979131997913
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 20492112049211
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 20808332080833
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 21919392191939
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 22030572203057
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 22711532271153
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 23263152326315
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - 24887822488782
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - JBC1780755JBC1780755
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - JC1646136JC1646136
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - JC1686015JC1686015
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - LV1462090LV1462090
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - LV1549837LV1549837
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - LV1581100LV1581100
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - NG1723973NG1723973
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - NG1741697NG1741697
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - NG1812618NG1812618
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - NG1834903NG1834903
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - NG1852175NG1852175
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - NG1886530NG1886530
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 - NG1726915NG1726915

      Références bibliographiques | 58 Disponible | Voir toutes les références

      Aperçu de la référence bibliographiqueEspèceNº PubMed
      hESC-derived Olig2(+) progenitors generate a subtype of astroglia with protective effects against ischaemic brain injury.
      Jiang, Peng, et al.
      Nat Commun, 4: 2196 (2013) 2013

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      23880652
      Expression of myelin basic protein in the human auditory nerve - an immunohistochemical and comparative study.
      Wei Liu,Marja Boström,Anders Kinnefors,Fred Linthicum,Helge Rask-Andersen
      Auris, nasus, larynx 39 2012

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      21601398
      Oligodendrocyte progenitor cells derived from mouse embryonic stem cells give rise to type-1 and type-2 astrocytes in vitro.
      Chen, Chen, et al.
      Neurosci. Lett., 523: 180-5 (2012) 2012

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      22781495
      Stable expression of FoxA1 promotes pluripotent P19 embryonal carcinoma cells to be neural stem-like cells.
      Difei Dong,Lei Meng,Qiqi Yu,Guixiang Tan,Miao Ding,Yongjun Tan
      Gene expression 15 2012

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      22783724
      Comparison of the D2 receptor regulation and neurotoxicant susceptibility of nigrostriatal dopamine neurons in wild-type and CB1/CB2 receptor knockout mice.
      Tyrell J Simkins,Kelly L Janis,Alison K McClure,Bahareh Behrouz,Samuel S Pappas,Andreas Lehner,Norbert E Kaminski,John L Goudreau,Keith J Lookingland,Barbara L F Kaplan
      Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology 7 2012

      Afficher le résumé
      22639229
      Arsenic Exposure Inhibits Myogenesis and Neurogenesis in P19 Stem Cells Through Repression of the β-Catenin Signaling Pathway.
      Gia-Ming Hong,Lisa J Bain
      Toxicological sciences : an official journal of the Society of Toxicology 129 2012

      Afficher le résumé
      22641621
      Tracing Synaptic Connectivity onto Embryonic Stem Cell-derived Neurons.
      Isabella Garcia,Longwen Huang,Kevin Ung,Benjamin R Arenkiel
      Stem cells (Dayton, Ohio) 30 2012

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      22996827
      Loss of neuronal potassium/chloride cotransporter 3 (KCC3) is responsible for the degenerative phenotype in a conditional mouse model of hereditary motor and sensory neuropathy associated with agenesis of the corpus callosum.
      Masoud Shekarabi,Randal X Moldrich,Sarah Rasheed,Adéle Salin-Cantegrel,Janet Laganière,Daniel Rochefort,Pascale Hince,Karine Huot,Rébecca Gaudet,Nyoman Kurniawan,Susana G Sotocinal,Jennifer Ritchie,Patrick A Dion,Jeffrey S Mogil,Linda J Richards,Guy A Rouleau
      The Journal of neuroscience : the official journal of the Society for Neuroscience 32 2012

      Afficher le résumé
      22423107
      Onecut transcription factors are required for the second phase of development of the A13 dopaminergic nucleus in the mouse.
      Agnès Espana,Frédéric Clotman
      The Journal of comparative neurology 520 2012

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      22102297
      Diverse effects of dimethyl sulfoxide (DMSO) on the differentiation potential of human embryonic stem cells.
      Rajarshi Pal,Murali Krishna Mamidi,Anjan Kumar Das,Ramesh Bhonde
      Archives of toxicology 86 2012

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      22105179

      Brochure

      Titre
      AXIS: Axon Investigation System

      Fiche technique

      Titre
      Anti-Tubulin, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) - Data Sheet

      Posters

      Titre
      Investigation of Axon Growth and Development Using Two Novel Assay Formats