EZGRT-89K | Human Ghrelin (total) ELISA

EZGRT-89K
96-well strip plate  
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      Overview

      Replacement Information

      Tableau de caractéristiques principal

      Analytes disponibles Espèces réactives Principales applications Méthodes de détection
      Ghréline (Totale)HumainELISAColorimétrie, Fluorescence
      Description
      Référence EZGRT-89K
      Description Human Ghrelin (total) ELISA
      Informations générales Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach.
      Références bibliographiques
      Informations produit
      Méthode de détection Colorimétrie Fluorescence
      Précision (%)
      • Intra-assay: Serum: 0.9–1.3% Plasma: 1.1–1.9%
      • Inter-assay: Serum: 6.2–7.8% Plasma: 5.2–7.7%
      Applications
      Application This Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.
      Principales applications
      • ELISA
      Notes opératoires Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
      Informations biologiques
      Spécificité Human Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
      Espèces réactives Humain
      Analytes disponibles
      • Ghréline (Totale)
      Code Entrez Gene
      Résumé Entrez Gene Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Symbole du gène
      • MTLRP
      • GHRL
      • ghrelin
      • obestatin
      Code UniProt
      Résumé UniProt FUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Exactitude
      • (Des-octanoyl Ghrelin) 112–119%
      • Serum: (Active Ghrelin) 88–91%
      • (Des-octanoyl Ghrelin) 101–107%
      • Plasma: (Active Ghrelin) 84–87%
      Informations physico-chimiques
      Sensibilité
      • 30 pg/mL (20 µL sample size)
      Linéarité de la dilution Serum: 95.7–104.7% Plasma: 95–117%
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Restrictions des ventes This product is not available for sale in Japan.
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Informations sur l'emballage
      Quantité 96-well strip plate
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      Protocols

      Title
      HUMAN TOTAL GHRELIN ELISA KIT

      FDS

      Langues
      English

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Immunohistochemical and quantitative analysis of ghrelin in Syzygium aromaticum.
      Aydin S, Dagli AF, Ozkan Y, Kendir Y, Sahin I, Aksoy A, Ozercan IH.
      Cell biology international 35 437-41 2011

      Afficher le résumé
      21029042
      Changes in glucose homeostasis after Roux-en-Y gastric bypass surgery for obesity at day three, two months, and one year after surgery: role of gut peptides.
      Falkén Y, Hellström PM, Holst JJ, Näslund E
      The Journal of clinical endocrinology and metabolism 96 2227-35. Epub 2011 May 4. 2011

      21543426
      The fermentable fibre inulin increases postprandial serum short-chain fatty acids and reduces free-fatty acids and ghrelin in healthy subjects.
      Tarini J, Wolever TM
      Applied physiology, nutrition, and metabolism = Physiologie 35 9-16. 2010

      Afficher le résumé
      20130660
      Alteration in chromogranin A, obestatin and total ghrelin levels of saliva and serum in epilepsy cases.
      Ersel Dag,Suleyman Aydin,Yusuf Ozkan,Fazilet Erman,Adile Ferda Dagli,Mehtap Gurger
      Peptides 31 2010

      Afficher le résumé
      20172008

      Brochure

      Titre
      ELISAs, RIAs & GyroMark HT Assays
      Immunoassay & Multiplex Kits: Analyte Quarterly, Volume 1, 2015

      Informations techniques

      Titre
      Quality Control Ranges: HUMAN TOTAL GHRELIN ELISA KIT - 96-Well Plate

      Fiche technique

      Titre
      Full Service Custom-built Assays