EZGLP1T-36K | Multi Species GLP-1 Total ELISA, EZGLP1T-36K

EZGLP1T-36K
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      Overview

      Tableau de caractéristiques principal

      Analytes disponibles Espèces réactives Principales applications
      Glucagon Like Peptide-1 R,H,M ELISA
      Description
      Référence EZGLP1T-36K
      Description Multi Species GLP-1 Total ELISA, EZGLP1T-36K
      Informations générales Glucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.
      Références bibliographiques
      Informations produit
      Spécificité The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.
      Précision (%)
      • Inter-assay:< 12%
      • Intra-assay: < 5%
      Applications
      Application This Multi Species GLP-1 Total ELISA, EZGLP1T-36K is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.
      Principales applications
      • ELISA
      Notes opératoires This is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.
      Informations biologiques
      Spécificité The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.
      Espèces réactives Rat Humain Souris
      Analytes disponibles
      • Peptide analogue au glucagon-1 (GLP-1)
      Réactivité croisée GLP-1 is conserved in almost all mammals.
      Code Entrez Gene
      Résumé Entrez Gene The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
      Symbole du gène
      • glucagon
      • GCG
      • GRPP
      • GLP1
      • GLP2
      Code UniProt
      Résumé UniProt FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
      SIZE: 180 amino acids; 20909 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
      PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
      SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
      MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
      Exactitude
      • Linearity 90 - 132%
      • Recovery: 90 - 110%
      Informations physico-chimiques
      Sensibilité
      • MinDC is 1.5 pM
      Gamme de la courbe standard
      • 4.1 – 1000 pM
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Informations relatives au stockage et à l'expédition
      Informations sur l'emballage
      Taille du matériau 96-well plate
      Informations sur le transport
      Information complémentaire
      Caractéristiques