ESG1107 | ESGRO® Leukemia Inhibitory Factor (LIF), 10 million units/1 mL

Undifferentiated ES cells cultured for five days in media containing EMD Millipore's ESGRO® (Cat. nos. ESG1106, ESG1107, LIF2005, LIF2010 & LIF2050). A concentration of 10 ng/mL is used for inhibition of differentiation. Catalog number SCR001 (ES Cell Characterization Kit) was used to stain the ES cell colony.
Undifferentiated ES cells cultured for five days in media containing EMD Millipore's ESGRO® (Cat. no
ESG1107
10⁷ units  1 mL
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      Overview

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      Offres spéciales

      High Quality. Validated. Reliable.

      EmbryoMax PMEF feeder cells, Millipore's convenient solution for ES cell culture.
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      Tableau de caractéristiques principal

      Principales applications Espèce Pureté
      Culture de cellules souchesMouseThe active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® Supplement is supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
      Description
      Référence ESG1107
      Famille de marques Chemicon®
      Synonymes mLIF Mouse LIF Murine LIF
      Nom de marque
      • Chemicon
      Description ESGRO® Leukemia Inhibitory Factor (LIF), 10 million units/1 mL
      Aperçu The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.
      Informations générales Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.
      Références bibliographiques
      Informations produit
      Format Purifié(e)
      Présentation ESGRO® Supplement is supplied in liquid form as 107 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
      Applications
      Application The ESGRO Leukemia Inhibitory Factor (LIF) supplement is an optimized formulation of recombinant mouse LIF (mLIF) for in vitro maintenance of mouse ES and iPS cells. Each vial contains 10^7 units.
      Principales applications
      • Culture de cellules souches
      Notes opératoires Applications for ESGRO® Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.

      SUGGESTED PROTOCOLS:

      ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U ESGRO® Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells(E3).

      At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® Supplement is sufficient for 1.0 L of tissue culture media.
      Informations biologiques
      Concentration 10 million units/1 mL
      Pureté The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® Supplement is supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
      Espèce Mouse
      Espèces réactives Souris
      Activité spécifique ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® Supplement, in 1.0mL of tissue culture medium, that induces the differentiation of 50% of M1 colonies(4). 10E7 units is equivalent to approximately 100 μg of pure protein, and is sufficient to treat 10L of ES cell culture media. <br /><br />Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 10<sup>8</sup> units/mg
      Code Entrez Gene
      Résumé Entrez Gene Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
      Symbole du gène
      • D-FACTOR
      • LIF
      • Emfilermin
      • CDF
      • MLPLI
      • HILDA
      Code UniProt
      Résumé UniProt FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
      SIZE: 202 amino acids; 22008 Da
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

        Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

        The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

        For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage ESGRO® Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO® Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO® Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.
      Informations sur l'emballage
      Quantité 10⁷ units
      Conditionnement 1 mL
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      Protocols

      Title
      General Experimental Outline for Targeting ES Cells

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      Certificats d'analyse

      TitreNuméro de lot
      ESGRO#174; Mouse LIF Medium Supplement - 20737382073738
      ESGRO#174; Mouse LIF Medium Supplement - 20115562011556
      ESGRO#174; Mouse LIF Medium Supplement - 20250272025027
      ESGRO#174; Mouse LIF Medium Supplement - 20322612032261
      ESGRO#174; Mouse LIF Medium Supplement - 20520742052074
      ESGRO#174; Mouse LIF Medium Supplement - 21522372152237
      ESGRO#174; Mouse LIF Medium Supplement - 21791142179114
      ESGRO#174; Mouse LIF Medium Supplement - 21975522197552
      ESGRO#174; Mouse LIF Medium Supplement - NG1951660NG1951660
      ESGRO#174; Supplement (107 Units) - DAM1739361DAM1739361
      ESGRO#174; Supplement (107 Units) - DAM1770436ADAM1770436A
      ESGRO#174; Supplement (107 Units) - DAM1835453DAM1835453
      ESGRO#174; Supplement (107 Units) - JBC1864978JBC1864978
      ESGRO#174; Supplement (107 Units) - JBC1872217JBC1872217
      ESGRO#174; Supplement (107 Units) - JBC1908448JBC1908448
      ESGRO#174; Supplement (107 Units) - NG1652954NG1652954
      ESGRO#174; Supplement (107 Units) - NG1654682NG1654682
      ESGRO#174; Supplement (107 Units) - NG1667894NG1667894
      ESGRO#174; Supplement - DAM1776540DAM1776540
      ESGRO#174; Supplement - DAM1821253DAM1821253
      ESGRO® Mouse LIF Medium -25712252571225
      ESGRO® Mouse LIF Medium Supplement -24611682461168
      ESGRO® Mouse LIF Medium Supplement -25102912510291
      ESGRO® Mouse LIF Medium Supplement -25610422561042
      MURINE ESGRO 107 - ME9EN5057ME9EN5057
      MURINE ESGRO 107 - ME9HN2210ME9HN2210

      Références bibliographiques | 14 Disponible | Voir toutes les références

      Aperçu de la référence bibliographiqueNº PubMed
      A kinase inhibitor screen identifies small-molecule enhancers of reprogramming and iPS cell generation.
      Li, Zhonghan and Rana, Tariq M
      Nat Commun, 3: 1085 (2012) 2012

      Afficher le résumé
      23011139
      A conditional knockout resource for the genome-wide study of mouse gene function
      Skarnes W. C. et al.
      Nature 474(7351) 337-342 2011

      Afficher le résumé
      21677750
      Optimization of Protocols for Derivation of Mouse Embryonic Stem Cell Lines from Refractory Strains, Including the Non Obese Diabetic Mouse.
      Davies T. J. & Fairchild P. J.
      Stem Cells Dev. Nov. 2011

      Afficher le résumé
      21933027
      Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
      Ettinger A. W. et al.
      Nat. Commun. 2 503 2011

      Afficher le résumé
      22009035
      Isolation and characterization of neural crest-derived stem cells from dental pulp of neonatal mice.
      Janebodin, Kajohnkiart, et al.
      PLoS ONE, 6: e27526 (2011) 2011

      Afficher le résumé
      22087335
      Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation.
      Ficz G. et al.
      Nature 473(7347) 398-402 2011

      Afficher le résumé
      21460836
      Using small molecules to improve generation of induced pluripotent stem cells from somatic cells
      Desponts C. & Ding S.
      Methods Mol. Biol. 636 207-218 2010

      Afficher le résumé
      20336525
      Novel hyperactive transposons for genetic modification of induced pluripotent and adult stem cells: a nonviral paradigm for coaxed differentiation.
      Belay, Eyayu, et al.
      Stem Cells, 28: 1760-71 (2010) 2010

      Afficher le résumé
      20715185
      Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2.
      Kanhere, Aditi, et al.
      Mol. Cell, 38: 675-88 (2010) 2010

      Afficher le résumé
      20542000
      Optimized mouse ES cell culture system by suspension growth in a fully defined medium.
      Andäng M. et al.
      Nat. Protoc, 3(6) 1013-1017 2008

      Afficher le résumé
      18536648

      Brochure

      Titre
      Murine Embryonic Stem Cell Culture Procedures & Protocols

      Informations techniques

      Titre
      Stem Cell Testing of PES Membrane Containing Stericup Filters
      The Genetically Modified Mouse: An Indispensable Tool for Disease Modeling

      Fiche technique

      Titre
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
      STEMCCA Lentivirus Reprogramming Kits

      FAQ

      QuestionRéponse
      Cause of Differation: Incubator settings Recommendation: Ensure that the CO2 incubator readings are correct at 37oC and 5% CO2.
      Cause of Differation: Concentration of ES cells Recommendation: Low confluency of ES cells can result in differentiation. ES cells should be plated at a minimum density of 1x106 cells / 100mm dish. Refer to the attached figures for illustrations of ES cell confluency. Refer to figs. 1, 2, 3, 4, and 5.
      Cause of Differation: Gelatin Recommendation: It is preferable to use cell culture grade gelatin at all times (even if using feeder layers) as the gelatin minimises surface differences on the tissue culture plates.
      Cause for the lack of chimeras: ES cell passage number Recommendation: In general; the best chimeras are generated from low passage number ES cells. If cell lines have been cultured for a high number of passages, it is recommended to go back to a lower passage frozen stock.
      Cause for the lack of chimeras: Time taken prior to microinjection Recommendation: It is recommend that ES cells be microinjected as soon as possible after removing them from the tissue culture plates, and not left on ice or room temperature for extended periods.
      Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend? Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.
      Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend? Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.
      Why are my mouse embryonic stem cells differentiating and what preventative measures do you recommend? Recommendation: Regular use of Penicillin / Streptomycin in media can often mask a low level contamination with agents such as mycoplasma. It is recommended to have your cell lines tested for mycoplasma on a periodic basis.