CYL3007 | PrecisION™ hKCNQ1/hminK-CHO Recombinant Stable Cell Line

CYL3007
2 mL  2 x 1 ml aliquots
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      Notes opératoires Espèce Cellules hôtes Protéine cible
      CHO-K1 cells stably expressing human KCNQ1 and human minK were analysed by patch-clamp and IonWorks. Over 80% of cells expressed >500 pA outward current (n=3180) with a mean current amplitude of 1050 pA. KvLQT1/minK currents were inhibited by standard blockers such as clotrimazole (pIC50 = 5.3±0.1; n=4) and linopirdine (pIC50 5.1±0.1; n=4).HumanChinese Hamster Ovarian K-1 cells (CHO K-1)hKCNQ1/hminK
      Description
      Référence CYL3007
      Famille de marques Upstate
      Nom de marque
      • Upstate
      • PrecisION
      Description PrecisION™ hKCNQ1/hminK-CHO Recombinant Stable Cell Line
      Aperçu Recombinant CHO-K1 cell line co-expressing the human KCNQ1 (potassium voltage-gated channel, KQT-like subfamily, member 1) and human minK (delayed rectifier potassium channel).

      Format:
      2 x 1 ml aliquots containing 180x 106cells/ml in 10% DMSO at passage 16.
      Alternate Names
      • LQT1
      • KCNQ1
      • JLNS1
      • ATFB1
      • SQT2
      • KCNA8
      • LQT
      • WRS
      • Kv1.9
      • KVLQT1
      • RWS
      • Kv7.1
      • KCNA9
      • LQTS
      • FLJ26167
      Informations générales KCNQ1 and mink form the slow delayed rectifier potassium current, IKs in the heart. This multimeric ion channel contributes to cardiac action potential repolarization and determines the heartbeat rate. Mutations in either KCNQ1 or minK that reduce IKs have been shown to cause long-QT syndrome (LQTS), a disorder of ventricular repolarization that can result in cardiac arrhythmia and sudden death. A well-recognized potential treatment for LQTS caused by reduction of IKs is to enhance functional activation of cardiac KCNQ1/minK channels.
      Références bibliographiques
      Informations produit
      Composants
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
      Présentation 2 x 1 ml aliquots
      Applications
      Application Human KCNQ1/minK ion channel stably expressed in CHO cells for use in electrophysiological assays.
      Notes opératoires CHO-K1 cells stably expressing human KCNQ1 and human minK were analysed by patch-clamp and IonWorks. Over 80% of cells expressed >500 pA outward current (n=3180) with a mean current amplitude of 1050 pA. KvLQT1/minK currents were inhibited by standard blockers such as clotrimazole (pIC50 = 5.3±0.1; n=4) and linopirdine (pIC50 5.1±0.1; n=4).
      Informations biologiques
      Espèce Human
      Code Entrez Gene
      Résumé Entrez Gene This gene encodes a protein for a voltage-gated potassium channel required for the repolarization phase of the cardiac action potential. The gene product can form heteromultimers with two other potassium channel proteins, KCNE1 and KCNE3. Mutations in this gene are associated with hereditary long QT syndrome, Romano-Ward syndrome, Jervell and Lange-Nielsen syndrome and familial atrial fibrillation. The gene is located in a region of chromosome 11 that contains a large number of contiguous genes that are abnormally imprinted in cancer and the Beckwith-Wiedemann syndrome. Two alternative transcripts encoding distinct isoforms have been described.
      Symbole du gène
      • LQT1
      • KCNQ1
      • JLNS1
      • ATFB1
      • SQT2
      • KCNA8
      • LQT
      • WRS
      • Kv1.9
      • KVLQT1
      • RWS
      • Kv7.1
      • KCNA9
      • LQTS
      • FLJ26167
      Cellules hôtes Chinese Hamster Ovarian K-1 cells (CHO K-1)
      Protéine cible hKCNQ1/hminK
      Sous-famille ciblée Potassium
      Code UniProt
      Résumé UniProt FUNCTION: SwissProt: P51787 # Probably important in cardiac repolarization. Associates with KCNE1 (MinK) to form the I(Ks) cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current. Muscarinic agonist oxotremorine-M strongly suppresses KCNQ1/KCNE1 current in CHO cells in which cloned KCNQ1/KCNE1 channels were coexpressed with M1 muscarinic receptors. May associate also with KCNE3 (MiRP2) to form the potassium channel that is important for cyclic AMP-stimulated intestinal secretion of chloride ions, which is reduced in cystic fibrosis and pathologically stimulated in cholera and other forms of secretory diarrhea.
      SIZE: 676 amino acids; 74699 Da
      SUBUNIT: Heteromultimer with KCNE1 (MinK) or KCNE3 (MiRP2).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Abundantly expressed in heart, pancreas, prostate, kidney, small intestine and peripheral blood leukocytes. Less abundant in placenta, lung, spleen, colon, thymus, testis and ovaries.
      DOMAIN: SwissProt: P51787 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
      DISEASE: SwissProt: P51787 # Defects in KCNQ1 are the cause of long QT syndrome type 1 (LQT1) [MIM:192500]; also known as Romano-Ward syndrome (RWS). Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to exercise or emotional stress. LQT1 inheritance is an autosomal dominant. & Defects in KCNQ1 are a cause of Jervell and Lange-Nielsen syndrome (JLNS) [MIM:220400]. JLNS is an autosomal recessive cardio-auditory syndrome characterized by a prolonged QT interval in the electrocardiogram and congenital deafness. & Defects in KCNQ1 are the cause of atrial fibrillation type 1 (ATFB1) [MIM:607554]. ATFB is characterized by rapid and irregular activation of the atrium. ATFB causes thromboembolism, tachycardia-mediated cardiomyopathy, heart failure and ventricular arrhythmia. & Defects in KCNQ1 are the cause of short QT syndrome type 2 (SQT2) [MIM:609621]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death.
      SIMILARITY: Belongs to the potassium channel family. KQT subfamily.
      MISCELLANEOUS: Mutagenesis experiments were carried out by expressing in Xenopus oocytes or COS-7 cells KCNQ1 mutants either individually (homomultimers) or in combination with both wild-type KCNQ1 (mut/wt homomultimers) and minK (heteromultimers).
      Type de lignées cellulaires
      • Canaux ioniques
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Mycoplasma Testing:
      The cell line has been screened to confirm the absence of Mycoplasma species
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Informations relatives au stockage et à l'expédition
      Informations sur l'emballage
      Quantité 2 mL
      Conditionnement 2 x 1 ml aliquots
      Informations sur le transport
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      Caractéristiques

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      Catégories

      Life Science Research > Cell Culture and Systems > Cell Lines > Ion Channel Cell Lines
      Life Science Research > Drug Discovery and Development > Ion Channel Cell Lines & Assays > Ion Channel Cell Lines