CC117 | Chicken Extracellular Chondroitin Sulfate Proteoglycans

Reducing SDS-PAGE of 6 μg of proteoglycan mixture (Catalog Number CC117) pretreated with chondroitinase (Lane 1) and without chondroitinase treatement (Lane 2). Major bands at 400 kDa and 250 kDa are visible.
Reducing SDS-PAGE of 6 μg of proteoglycan mixture (Catalog Number CC117) pretreated with chondroitin
CC117
100 µg  
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      Overview

      Replacement Information

      Tableau de caractéristiques principal

      Principales applications
      Culture cellulaire
      Description
      Référence CC117
      Famille de marques Chemicon®
      Nom de marque
      • Chemicon
      Description Chicken Extracellular Chondroitin Sulfate Proteoglycans
      Aperçu DESCRIPTION/COMPOSITION:CC117 contains a mixture of large, extracellular chondroitin sulfateproteoglycans isolated from embryonic chicken brain. Amino acid sequence analyses and immunological studies have demonstrated that the major components of this mixture are neurocan, phosphacan, versican, and aggrecan. Following chondroitinase treatment to remove the glycosaminoglycan portion of these proteoglycans, the major components of this preparation resolved on SDS gels are 250 kDa core proteins representing neurocan and 400 kDa core proteins representing aggrecan, phosphacan, and versican.

      *Quantity: Protein as determined using the bicinchoninic acid (BCA) assay. Note that 100 μg is a measurement of core polypeptides. Components of this mixture contain core polypeptide plus attached glycosaminoglycans
      Informations générales Aggrecan, versican, and neurocan are members of the family of hyaluronic acid-binding proteoglycans and therefore each contains hyaluronic acid binding regions, Ig-like domains, EGF like domains, lectin-like domains, and complement regulatory-like domains. Phosphacan is distinct; it contains a region of similarity to carbonic anhydrase, a fibronectin type III domain, and is a splice variant of the transmembrane protein receptor tyrosine phosphatase equivalent to its extracellular portion.

      Although aggrecan was traditionally thought of as a cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four of these proteoglycans have broad yet distinctive distribution patterns inearly embryos. The presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and are capable of inhibiting both cell-ECM and cell-cell adhesion.
      Références bibliographiques
      Informations produit
      Présentation Provided as a liquid in PBS, containing no preservatives.
      Applications
      Principales applications
      • Culture cellulaire
      Notes opératoires CC117 has been used successfully with rat and mouse brain slice culture systems.
      Informations biologiques
      Concentration 1 mg/mL
      Pureté Mixture of large, extracellular chondroitin sulfate proteoglycans (CSPGs) purified from embryonic chicken brains by chromatrographic methods. By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of each CSPG contained in the mixture is not quantified, however the virtually all of the CSPGs present in the source brains are collected.

      HOMOGENEITY:

      By PAGE analysis material is 95% pure, and migrates as a large HMW (<400kDa) mass under reduced conditions. The precise amounts of CSPG species has not been determined.
      Source Isolated from whole 12-14 day old embryonic chicken brains.
      Espèce Chicken
      Code UniProt
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Store at -20°C for up to 12 months. Avoid repeated freeze/thaw.
      Informations sur l'emballage
      Quantité 100 µg
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      Certificats d'analyse

      TitreNuméro de lot
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2382596 2382596
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2428484 2428484
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2459007 2459007
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 1990900 1990900
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2041535 2041535
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2049212 2049212
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2061429 2061429
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2136990 2136990
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2283321 2283321
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2300455 2300455
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - 2362870 2362870
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE 2506342 2506342

      Références bibliographiques

      Aperçu de la référence bibliographiqueEspèceNº PubMed
      Keratan sulfate proteoglycan phosphacan regulates mossy fiber outgrowth and regeneration.
      Butler, Christy D, et al.
      J. Neurosci., 24: 462-73 (2004) 2004

      Afficher le résumé
      14724244
      Hyaluronan-associated adhesive cues control fiber segregation in the hippocampus.
      Förster, E, et al.
      Development, 128: 3029-39 (2001) 2001

      Rat11532924
      Receptor-mediated adhesive and anti-adhesive functions of chondroitin sulfate proteoglycan preparations from embryonic chicken brain.
      Ernst, H, et al.
      J. Cell. Sci., 108 ( Pt 12): 3807-16 (1995) 1995

      Afficher le résumé
      8719887
      The interaction of the retina cell surface N-acetylgalactosaminylphosphotransferase with an endogenous proteoglycan ligand results in inhibition of cadherin-mediated adhesion.
      Balsamo, J, et al.
      J. Cell Biol., 129: 1391-401 (1995) 1995

      Afficher le résumé
      7775582

      Fiche technique

      Titre
      EXTRACELLULAR CHONDROITIN SULFATE PROTEOGLYCAN MIXTURE - Data Sheet