Cellules à agitation

Pressure-based sample concentration



Guide d'achat

Cellules à agitation Supprimer le tri & les filtres
Référenceicon Descriptionicon Conditionnementicon
5121Cellule à agitation modèle 8010, 10 ml1 Prix & Disponibilité
5122Cellule à agitation modèle 8050, 50 ml1 Prix & Disponibilité
5123Cellule à agitation modèle 8200, 200 ml1 Prix & Disponibilité
5124Cellule à agitation modèle 8400, 400 ml1 Prix & Disponibilité
5125Cellule à agitation modèle 8003, 3 ml1 Prix & Disponibilité

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Kits de pièces détachées Supprimer le tri & les filtres
Référenceicon Descriptionicon Conditionnementicon
8000SCPKITSmall Replacement Parts for 8000 Series Stirred Cells1 kit Prix & Disponibilité
8003SCPKITLarge Replacement Parts for 8003 Series Stirred Cells1 kit Prix & Disponibilité
8010SCPKITLarge Replacement Parts for 8010 Series Stirred Cells1 kit Prix & Disponibilité
8050SCPKITLarge Replacement Parts for 8050 Series Stirred Cells1 kit Prix & Disponibilité
8200SCPKITLarge Replacement Parts for 8200 Series Stirred Cells1 kit Prix & Disponibilité
8400SCPKITLarge Replacement Parts for 8400 Series Stirred Cells1 kit Prix & Disponibilité

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    Fiche technique

    Series 8000 Stirred Cells and Ultrafiltration Membranes

    Références bibliographiques | 35 Disponible | Voir toutes les références

    Aperçu de la référence bibliographiqueApplication
    Electrokinetic characterisation of ultrafiltration membranes by streaming potential, electroviscous effect, and salt retention
    Huisman IH (REPRINT) ; Pradanos P; Hernandez A
    Journal of membrane science 2000, V178, N1-2, p55-64 2000 2000

    Continuous production of oligosaccharides from whey using a membrane reactor
    Foda MI; Lopez Leiva M (reprint)
    Process Biochemistry 2000, V35, N6, P581-587 SN- 0032-9592 2000

    Integrated process for the removal of emulsified oils from effluents in the steel industry
    Benito JM; Rios G; Gutierrez B; Pazos C; Coca J (REPRINT)
    Separation Science and Technology 1999, V34, N15, P3031-3043 SN- 0149-6395 1999

    Industrial QC
    Acrylamide production in an ultrafiltration-membrane bioreactor using cells of Brevibacterium imperialis CBS 489-74
    Cantarella M (reprint) ; Spera A; Cantarella L; Alfani F ; Journal of membrane science, v147, N2, p279-290 1998
    Journal of membrane science, v147, N2, p279-290 1998 1998

    Enzyme Assay
    In vitro and in vivo grafting of xeno pig fetal liver fragments using ultrafiltration membrane
    Kanai N.; Morita N.; Munkhbat B.; Gansuvd B.; Hagihara M.; Nagamachi Y.;Tsuji K.
    Cell Transplantation SO- 7/4 (417-420) 1998 1998

    Cell Culture
    Metabolic screening using on-line ultrafiltration mass spectrometry
    Van Breemen R.B.; Nikolic D.; Bolton J.L.
    Drug Metabolism and Disposition , 26/2 (85-90) 1998 1998

    Performance Characteristics of Waste Oil Emulsion Treatment by Ultrafiltration
    Lin, S. H.; Lan, W. J. Yuan Ze University, Taoyuan, Taiwan
    J Environ Sci Health-Toxic Hazard Subst Environ Eng 1998,SO- vA33, n3, p385(20) 1998

    General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification
    Lin, S.-C. Chen, Y.-C. Lin, Y.-M.
    J. Chromatogr., A ; Journal of Chromatography, A v. 825 pg 149-159 6 Nov 1998 1998

    Filtration 15 nm: Aspects Techniques
    Chtourou S,Sang thrombose vaisseaux, 21-27, 1998
    Sang thrombose vaisseaux, 21-27, 1998 1998

    Distribution of trace elements associated with dissolved compounds (<0.45 .mu.m-1 nm) in freshwater using coupled (frontal cascade) ultrafiltration and chromatographic separations
    Pham, M. K. Garnier, J.-M.
    Environmental Science and Technology, v32, pg. 440-449, 15 Feb. 1998 1998


    Can I use compressed air instead of nitrogen to pressurize the cell? We don't recommended it because air may cause pH shifts (C02 adsorption) or oxidation of product.
    Can the membranes for the Stirred Cells be reused? Yes, the membranes can be reused several times given they are used, cleaned, and stored properly. The operating instructions for the Ultrafiltration Membranes (literature number 99101) includes a restoration protocol for the membranes specific to each type. Also, under the operating procedures section is the proper method for usage and storage.
    Can the Stirred Cell products be sanitized with Sodium Hypochlorite? Yes. We recommend 100 ppm (parts per million) of Sodium Hypochlorite for 30 min.
    Does tubing come with the stirred cell? Yes, the following tubing comes with the stirred cell: a. Four feet of 1/4" pressure tubing for pressurizing the stirred cell. b. Four inches of permeate tubing.
    How do I sterilize my stirred cell? The 8000 series can be sterilized using any one of the following: 70% Ethanol 70% Isopropanol 5% Formaldehyde <200 ppm NaOCl (sanitize) The Model 2000 can be sterilized using either of the following: 5% Formaldehyde <200 ppm NaOCL (sanitize)
    What concentration can I use with the Stirred Cells? All standard stirred cells operate within the following range:
    • Minimum initial concentration 0.05g%
    • Maximum Final Concentration 10g%
    What could be wrong if the stirrer assembly in the Stirred Cell won't spin? The stir bar won't spin unless the stirred cell is pressurized.
    Are there cases where a stirred cell outperforms a crossflow type system? Yes, at protein levels below 10 ug/ml. Also, pressure has a greater effect on flux as protein concentrations decrease. Most laboratory tangential flow systems operate at low pressure (20-25 psi), due to peristaltic pump limitations. Stirred cells do not require a pump for operation. They are normally pressurized to 55 psi.
    Where can I order the filtrate tubing to the Stirred Cell (Cat. No. 4500430) when it is no longer offered at Millipore? Cat. #4500430, TUBING (TYGON R-3603, 5/32X3/32), can be purchased through Fisher Scientific (800-766-7000) or Cole Palmer (800-323-4340)
    What fitting do I need to connect the stirred cell to a regulator? Tubing adapter kit (product number 6022) provides two fittings that should fit any regulator.
    Can the stirred cell be autoclaved with a membrane in place? Yes, but only with the YM membrane. Put a small amount of buffer in the device to keep the membrane wet, tighten the bottom then loosen 1/4 turn. Remove any tubing and fittings, they are not autoclavable.
    What pressures are Stirred Cells operated at? The maximum operating pressures for Models 8003, 8010, 8050, 8200 & 8400 is 75psi and Model 2000 is 100 psi. The recommended operating pressure for all Stirred Cells is 55 psi and <10psi when using a 100,000 MWCO membrane or greater.
    How do I know the membrane in my stirred cells is in correctly? The glossier side of the membrane should be facing up.
    How do I remove the stirred cell after I've vented the pressure? Push the cap down, then slide the cell out from the retaining stand.
    I bought some replacement bodies for my old style stirred cell. They did not fit properly. Has there been a product change? In March of 1990 the stirred cell bases were changed to a white plastic molded part from a black metal part. Bodies compatible with the black metal base are not compatible with the white plastic base. When ordering a replacement body you will also need to order a new base. Here are the catalogue numbers for the affected products:
    CAT #DescriptionBodyBase
    5125Model 8003153270521000
    5121Model 8010 153270121000
    5122Model 8050153270220987
    5123Model 8200153270321011
    5124Model 8400153270421003
    In what order do I assemble the parts to the tube fitting assembly? Assemble in the following order: First, the hexagonal nut slides on to the red pressure tubing. The metal grab ring with flange facing away from hex nut fits into hex nut. The white plastic spacer with countersink surface towards grab ring is next. Finally, the o-ring is placed onto the end of the assembly.
    What comes with the 8000 series stirred cells? Everything except the magnetic stirrer, pressure source and the membrane.
    What is the minimum volume that the stirred cells can concentrate to? Amicon stirred cells can concentrate down to the minimum volumes listed below:
    ModelCell Capacity (ml) Minimum Vol
    800330.05 ml
    801010 1 ml
    805050 2.5 ml
    82002005 ml
    840040010 ml
    20002000 50 ml
    What products should I purchase to continuously diafilter? Pressurized Reservoir:
    Model 8003 - RC800 (product no. 6028),
    1/4" pressure tubing (product no. XXPE00010)
    Concentration/Dialysis Selector CDS10 (product no. 6003)
    To what protein concentration can the solution be concentrated to using a stirred cell? Practical limit is 10% by weight at room temp and 55psi. Slightly higher if solution can be warmed.
    Which membranes should I use in my stirred cell? The following list indicates the appropriate disc size for the various stirred cells listed below:
    Model Membrane Diameter (mm)
    8003 25
    8010 25
    8050 43
    8200 62
    8400 76
    2000 150
    Are the Stirred Cells autoclavable? Yes, stirred cells can be autoclaved at 121 C for 30 minutes. It is important, however, to tighten the base the loosen 1/4 turn before autoclaving. The tubing and the tubing connector are not autoclavable.
    Do you sell parts for the Stirred Cells individually? What is the part number and price? Yes, the parts lists for the Model 8000 Stirred Cells can be found in the Stirred Ultrafiltration User's Guide P99228. The parts list for the Model 2000 High Output Stirred Cell can be found in the user's guide P99109. Parts for the older style Stirred Cells (Model 12, 52, 202 or 402) are no longer being replaced.
    I don't have any flow in my stirred cell. What happened? a. Cell is not pressurized.
    b. Membrane upside down. Glossy side should face up.
    c. Plastic sheet not removed from membrane (XM-300 only),
    d. Solution may be too concentrated (viscous).
    e. Check that the membrane holder and filtrate port is not blocked.
    Should I use the Ultracel regenerated cellulose membrane or the Biomax membrane in my TFF system? It depends on the nature of your process volume and the process speed required. The Ultracel membrane is a lower protein binding membrane that should be used in low protein loading applications(<20 g/m2). The Biomax membrane is a faster flowing membrane that should be used in high protein loading applications (>20 g/m2). Ultracel is less tolerant to extreme pH conditions than the Biomax, but the Ultracel is more compatible with organic solvents.
    What products and catalog numbers are required for the diafiltration set-up? The diafiltration set-up requires the following:

    1. Stirred Cell, for example catalogue number 5121 - Model 8010 (which uses 25mm membrane discs).
    2. Magnetic stirring table.
    3. Reservoir or dispensing pressure vessel. For example, catalogue number 6028 - RC800 Reservoir (800mL) or catalogue number XX6700P01 - 3.785 liter Dispensing Pressure Vessel (this will require relief valve, hose connectors, etc.)
    4. CDS10 Concentration/Dialysis Selector, catalogue number 6003.
    5. Pressure tubing to connect the system to a pressure source. XXPE00010 is 10 feet of 1/4" OD polyethylene tubing.
    6. Pressure source

    Manuels d'utilisation

    Stirred Ultrafiltration Cell User Guide

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    Reagents, Chemicals and Labware > Lab Filtration > Stirred Ultrafiltration Units


    Les cellules à agitation Amicon procurent des débits élevés avec les solutions contenant jusqu'à 10 % de macrosolutés. La même unité permet de réaliser une concentration rapide ou un dessalage suivi d'une concentration. Une récupération totale de l'échantillon est en général possible en utilisant la configuration de diafiltration présentée ci-dessous.

    L'agitation douce par barreau aimanté minimise la formation d'une couche de polarisation et la dénaturation par cisaillement. Toutes les cellules à agitation peuvent être autoclavées.

    Configuration du mode de diafiltration et fonctionnement avec vanne de sélection Amicon, cellule à agitation et réservoir annexe.<br />1 - Ultrafiltrat, 2 - Matériel d'ultrafiltration, 3 - Arrivée de pression, 4 - Vanne de sélection, 5 - Entrée, 6 - Sortie, 7 - Solution d'alimentation, 8 - Réservoir

    Configuration du mode de diafiltration et fonctionnement avec vanne de sélection Amicon, cellule à agitation et réservoir annexe.
    1 - Ultrafiltrat, 2 - Matériel d'ultrafiltration, 3 - Arrivée de pression, 4 - Vanne de sélection, 5 - Entrée, 6 - Sortie, 7 - Solution d'alimentation, 8 - Réservoir

    Stirred Cells

    Stirred Cells

    Stirred Cells

    Stirred Cells

    Stirred Cells

    Stirred Cells

    Couvercle et pièces de raccordement Nylon
    Réservoir et support de membrane Polysulfone
    Barreau aimanté Acétal,
    Joints toriques Elastomère de silicone
    Tuyau pour utilisation sous pression Polyéthylène
    Tuyau à filtrat Tuyau en Tygon®
    Etrier Aluminium
    anodisé ou nylon
    Cellule à agitation Modèle 8003, réf. 5125 Modèle 8010, réf. 5121 Modèle 8050, réf. 5122 Modèle 8200, réf. 5123 Modèle 8400, réf. 5124
    Volume traité max., ml 3 10 50 200 400
    Volume traité min., ml 0,075 1,0 2,5 5,0 10,0
    Diamètre de la
    membrane, mm
    25 25 44,5 63,5 76
    Surface de membrane efficace, cm2 0,9 4,1 13,4 28,7 41,8
    Volume mort, ml 0,07 0,2 0,5 1,2 1,5
    Hauteur, cm 7,7 7,7 9,8 12,8 15,5
    Base, cm 6 x 6 6 x 6 7 x 7 9 x 9 11 x 11
    Poids, kg 0,1 0,1 0,2 0,4 0,6