AB5535 | Anti-Sox9 Antibody

Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (FFPE) murine embryonic developing bone (Panels A&B) and adult murine cartilage (Panel C) tissue sections were processed using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:500 dilution of Cat. No. AB5535, Anti-Sox9. Reactivity was detected using an Anti-Rabbit secondary antibody and HRP-DAB. Positive nuclear staining was observed in the tissue sections.
Immunohistochemistry Analysis:
Representative lot data.
Formalin Fixed Paraffin Embedded (
AB5535
100 µg  
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      Overview

      Replacement Information

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      Tableau de caractéristiques principal

      Espèces réactives Principales applications Hôte Format Type d'anticorps
      R, Humain, SourisWB, ImmunohistochimieLapinPurifié(e) par affinitéAnticorps polyclonal
      Description
      Référence AB5535
      Replaces AB5809
      Famille de marques Chemicon®
      Nom de marque
      • Chemicon
      Description Anti-Sox9 Antibody
      Informations générales Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.
      Références bibliographiques
      Informations produit
      Format Purifié(e) par affinité
      Contrôle
      • Embryonic tissue, Adult chondrocytes.
      Présentation Purified rabbit polyclonal in buffer containing 0.1M Tris -Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4.
      Applications
      Application Anti-Sox9 Antibody is a well characterized affinity purified Rabbit Polyclonal Antibody that reliably detects Transcription Factor Sox-9. This highly published antibody has been validated in IHC & WB.
      Principales applications
      • Western Blotting
      • Immunohistochimie
      Notes opératoires Western Blot:
      1:500 dilution of a previous lot ~65kDa band

      EMSA supershift

      Optimal working dilutions must be determined by the end user.
      Informations biologiques
      Espèces d'immunogène Synthetic peptide from human Sox9.
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hôte Lapin
      Spécificité Recognizes Sox9.
      Espèces réactives Rat Humain Souris
      Species Reactivity Note Human, rat and fetal mouse. Other species have not yet been tested.
      Type d'anticorps Anticorps polyclonal
      Code Entrez Gene
      Résumé Entrez Gene The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
      Symbole du gène
      • SRA1
      • SOX9
      • CMPD1
      • CMD1
      Méthode de purification Immunoaffinity purified
      Code UniProt
      Résumé UniProt FUNCTION: SwissProt: P48436 # Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
      SIZE: 509 amino acids; 56137 Da
      SUBCELLULAR LOCATION: Nucleus (Potential).
      DISEASE: SwissProt: P48436 # Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male- to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
      SIMILARITY: SwissProt: P48436 ## Contains 1 HMG box DNA-binding domain
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Routinely evaluated by Western Blot on L6 cell lysates.

      Western Blot Analysis: 1:2000 dilution of this lot detected SOX-9 on 10 μg of L6 cell lysate
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
      Informations sur l'emballage
      Quantité 100 µg
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      FDS

      Langues
      Deutschland

      Certificats d'analyse

      TitreNuméro de lot
      Anti-Sox9 - 23839732383973
      Anti-Sox9 - 24247512424751
      Anti-Sox9 - 24628602462860
      Anti-Sox9 - 19697021969702
      Anti-Sox9 - 19973951997395
      Anti-Sox9 - 20313302031330
      Anti-Sox9 - 20659992065999
      Anti-Sox9 - 21671532167153
      Anti-Sox9 - 22626792262679
      Anti-Sox9 - 23618182361818
      Anti-Sox9 - 24940292494029
      Anti-Sox9 - LV1478872LV1478872
      Anti-Sox9 - LV1580835LV1580835
      Anti-Sox9 - LV1679322LV1679322
      Anti-Sox9 - LV1738269LV1738269
      Anti-Sox9 - LV1762669LV1762669
      Anti-Sox9 - NG1839530NG1839530
      Anti-Sox9 - NG1884650NG1884650
      Anti-Sox9 - NG1901550NG1901550
      Anti-Sox9 - NG1922963NG1922963
      Anti-Sox9 -25103212510321
      Anti-Sox9 -25522812552281
      Anti-Sox9 -25857852585785
      RABBIT ANTI-POTASSIUM CHANNEL MAXI K (BKCa) - 24946102494610

      Références bibliographiques | 54 Disponible | Voir toutes les références

      Aperçu de la référence bibliographiqueNº PubMed
      Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer.
      Saha, SK; Parachoniak, CA; Ghanta, KS; Fitamant, J; Ross, KN; Najem, MS; Gurumurthy, S; Akbay, EA; Sia, D; Cornella, H; Miltiadous, O; Walesky, C; Deshpande, V; Zhu, AX; Hezel, AF; Yen, KE; Straley, KS; Travins, J; Popovici-Muller, J; Gliser, C; Ferrone, CR; Apte, U; Llovet, JM; Wong, KK; Ramaswamy, S; Bardeesy, N
      Nature 513 110-4 2014

      Afficher le résumé
      25043045
      GATA4 and GATA6 control mouse pancreas organogenesis.
      Manuel Carrasco,Irene Delgado,Bernat Soria,Francisco Mart,Anabel Rojas
      The Journal of clinical investigation 122 2012

      Afficher le résumé
      23006330
      Testicular development in mice lacking receptors for follicle stimulating hormone and androgen.
      Peter J O'Shaughnessy,Ana Monteiro,Margaret Abel
      PloS one 7 2012

      Afficher le résumé
      22514715
      Regulation of human lung alveolar multipotent cells by a novel p38α MAPK/miR-17-92 axis.
      Feride Oeztuerk-Winder,Anna Guinot,Anna Ochalek,Juan-Jose Ventura
      The EMBO journal 31 2012

      Afficher le résumé
      22828869
      Sox9 directs hypertrophic maturation and blocks osteoblast differentiation of growth plate chondrocytes.
      Peter Dy,Weihuan Wang,Pallavi Bhattaram,Qiuqing Wang,Lai Wang,R Tracy Ballock,Véronique Lefebvre
      Developmental cell 22 2012

      Afficher le résumé
      22421045
      BAC-Dkk3-EGFP transgenic mouse: an in vivo analytical tool for Dkk3 expression.
      Yuki Muranishi,Takahisa Furukawa
      Journal of biomedicine & biotechnology 2012 2012

      Afficher le résumé
      22910798
      Isolation, differentiation, and characterisation of skeletal stem cells from human bone marrow in vitro and in vivo.
      Rahul S Tare,Peter D Mitchell,Janos Kanczler,Richard O C Oreffo
      Methods in molecular biology (Clifton, N.J.) 816 2012

      Afficher le résumé
      22130924
      Oncogenicity of the developmental transcription factor Sox9.
      Ander Matheu,Manuel Collado,Clare Wise,Lorea Manterola,Lina Cekaite,Angela J Tye,Marta Canamero,Luis Bujanda,Andreas Schedl,Kathryn S E Cheah,Rolf I Skotheim,Ragnhild A Lothe,Adolfo López de Munain,James Briscoe,Manuel Serrano,Robin Lovell-Badge,Adolfo L
      Cancer research 72 2012

      Afficher le résumé
      22246670
      Slug and Sox9 cooperatively determine the mammary stem cell state.
      Guo, Wenjun, et al.
      Cell, 148: 1015-28 (2012) 2012

      Afficher le résumé
      22385965
      The maturation of photoreceptors in the avian retina is stimulated by thyroid hormone.
      Fischer AJ, Bongini R, Bastaki N, Sherwood P
      Neuroscience 178 250-60. Epub 2011 Jan 19. 2011

      Afficher le résumé Article en texte intégral
      21256198

      Brochure

      Titre
      SNAP i.d. 2.0 System Brochure

      Fiche technique

      Titre
      Anti-Sox9 - Data Sheet