AB5204-50UL | Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I

AB5204-50UL
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      Overview

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      Tableau de caractéristiques principal

      Espèces réactives Principales applications Hôte Format Type d'anticorps
      R, SourisWB, ImmunohistochimieLapinPurifié(e) par affinitéAnticorps polyclonal
      Description
      Référence AB5204-50UL
      Famille de marques Chemicon®
      Synonymes Nav1.1; SCN1A
      Nom de marque
      • Chemicon
      Description Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I
      Références bibliographiques
      Informations produit
      Format Purifié(e) par affinité
      Spécificité Recognizes type I alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

      SPECIES REACTIVITIES: It is expected that the antibody may also react with human due to sequence homology. Other species have not been tested.
      Contrôle
      • CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      Présentation Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Applications
      Application Detect Sodium Channel using this Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I validated for use in IH & WB.
      Principales applications
      • Western Blotting
      • Immunohistochimie
      Notes opératoires All procedures that are going to receive a full-length protein should be performed at 4C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).

      Western blot: 0.5 to 1.5 μg/mL (1:200-1:600) using ECL

      Immunohistochemistry on rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Informations biologiques
      Espèces d'immunogène Purified peptide SP11 (Noda et al. 1986) from 465-481 of alpha subunit of rat type I voltage-gated sodium channel (VGSC), conjugated to KLH with gluteraldehyde (Harlow & Lane 1988).
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hôte Lapin
      Spécificité Recognizes type I alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

      SPECIES REACTIVITIES: It is expected that the antibody may also react with human due to sequence homology. Other species have not been tested.
      Espèces réactives Rat Souris
      Type d'anticorps Anticorps polyclonal
      Code Entrez Gene
      Résumé Entrez Gene The vertebrate sodium channel is a voltage-gated ion channel essential for the generation and propagation of action potentials, chiefly in nerve and muscle. Voltage-sensitive sodium channels are heteromeric complexes consisting of a large central pore-forming glycosylated alpha subunit and 2 smaller auxiliary beta subunits. Functional studies have indicated that the transmembrane alpha subunit of the brain sodium channels is sufficient for expression of functional sodium channels (Goldin et al., 1986 [PubMed 2429308]; Isom, 2002 [PubMed 11779698]).[supplied by OMIM]
      Symbole du gène
      • GEFSP2
      • SCN1A
      • SCN1
      • HBSCI
      • Nav1.1
      • FEB3
      • NAC1
      • SMEI
      Code UniProt
      Résumé UniProt FUNCTION: SwissProt: P35498 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
      SIZE: 2009 amino acids; 228972 Da
      SUBUNIT: The sodium channel consists of a large polypeptide and 2- 3 smaller ones. This sequence represents a large polypeptide.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DOMAIN: SwissProt: P35498 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
      DISEASE: SwissProt: P35498 # Defects in SCN1A are the cause of generalized epilepsy with febrile seizures plus type 2 (GEFS+2) [MIM:604233]. This autosomal dominant disorder is characterized by febrile seizures in children and afebrile seizures in adults. Penetrance is incomplete and a large intrafamilial variability of the phenotype is observed. & Defects in SCN1A are a cause of severe myoclonic epilepsy in infancy (SMEI) [MIM:607208]; also called Dravet syndrome. SMEI is a severe form of generalized epilepsy with febrile seizures. It is a rare disorder characterized by normal development before onset, seizures beginning in the first year of life in the form of generalized or unilateral febrile clonic seizures, secondary appearance of myoclonic seizures, and occasionally partial seizures. It is associated with ataxia, slowed psychomotor development, and mental decline. & Defects in SCN1A are the cause of familial hemiplegic migraine 3 (FHM3) [MIM:609634]. FHM3 is an autosomal dominant severe subtype of migraine with aura characterized by some degree of hemiparesis during the attacks. The episodes are associated with variable features of nausea, vomiting, photophobia and phonophobia. Age at onset ranges from 6 to 15 years. Some patients may manifest seizures during infancy. & Defects in SCN1A are the cause of familial febrile convulsions 3 (FEB3) [MIM:604403]; also known as familial febrile seizures 3. Febrile convulsions affect 5-12% of infants and children up to 6 years of age. There is epidemiological evidence that febrile seizures are associated with subsequent afebrile and unprovoked seizures in 2% to 7% of patients. Inheritance pattern is autosomal dominant.
      SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Maintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Informations sur l'emballage
      Taille du matériau 50 µL
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

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      Certificats d'analyse

      TitreNuméro de lot
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL (Nav1.1) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2117982 2117982
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL (Nav1.1) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2135072 2135072
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL (Nav1.1) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2302145 2302145
      Rabbit Anti-Brain Type I Voltage 2462788

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Spontaneous epileptiform discharges in a mouse model of Alzheimer\'s disease are suppressed by antiepileptic drugs that block sodium channels.
      Ziyatdinova S, Gurevicius K, Kutchiashvili N, Bolkvadze T, Nissinen J, Tanila H, Pitkänen A
      Epilepsy research 94 75-85. 2011

      Afficher le résumé
      21300523
      Elevated expression of type II Na+ channels in hypomyelinated axons of shiverer mouse brain.
      Westenbroek, R E, et al.
      J. Neurosci., 12: 2259-67 (1992) 1992

      Afficher le résumé
      1318958
      Tissue-specific expression of the RI and RII sodium channel subtypes.
      Gordon, D, et al.
      Proc. Natl. Acad. Sci. U.S.A., 84: 8682-6 (1987) 1987

      Afficher le résumé
      2446328
      Expression of functional sodium channels from cloned cDNA.
      Noda, M, et al.
      Nature, 322: 826-8 (1986) 1986

      Afficher le résumé
      2427955

      Fiche technique

      Titre
      RABBIT ANTI-BRAIN TYPE I VOLTAGE GATED SODIUM CHANNEL (Nav1.1) AFFINITY PURIFIED POLYCLONAL ANTIBODY

      Produits & Applications associés

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       Référence Description  
      AB5204 Anti-Sodium Channel Antibody, Voltage Gated, Brain Type I Prix & Disponibilité

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies