AB1504 | Anti-Glutamate receptor 1 Antibody

Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded rat brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:300 dilution of Cat. No. AB1504, Anti-Glutamate receptor 1. Reactivity was detected using the IHC-Select Detection Kit (Cat. No. DAB050). Staining pattern appears as cytoplasmic.
Immunohistochemistry Analysis:
Representative lot data.
Paraffin-embedded rat brain tissu
AB1504
200 µL  
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      Tableau de caractéristiques principal

      Espèces réactives Principales applications Hôte Format Type d'anticorps
      Eq,R,M,H IP,WB,IH(P) Rb Affinity Purified Polyclonal Antibody
      Description
      Référence AB1504
      Synonymes glutamate receptor, ionotropic, AMPA 1 AMPA 1 AMPA-selective glutamate receptor 1 Glutamate receptor ionotropic, AMPA 1
      Description Anti-Glutamate receptor 1 Antibody
      Informations générales Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. GluR1 is believed to mediate calcium influx, and has a vital function in synaptic plasticity related to learning. Reduction in GluR1 has been caused by FMR1 gene mutation and contributes to fragile X syndrome. Changes in expression of GluR1 receptors on dopamine neurons have been correlated with sensitization of drugs of abuse.
      Références bibliographiques
      Informations produit
      Format Purifié(e) par affinité
      Spécificité This antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.
      Contrôle
      • Mouse brain lysate
      Présentation Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      Application This Anti-Glutamate receptor 1 Antibody is validated for use in WB, IH(P), IP for the detection of Glutamate receptor 1.
      Principales applications
      • Immunoprécipitation
      • Western Blotting
      • Immunohistochimie (parafine)
      Notes opératoires Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Glutamate receptor 1 (GluR1) in rat brain tissue.
      Informations biologiques
      Espèces d'immunogène KLH-conjugated linear peptide corresponding to human Glutamate receptor 1 at the cytoplasmic domain.
      Épitope Cytoplasmic domain
      Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
      Hôte Lapin
      Spécificité This antibody recognizes Glutamate receptor 1 at the cytoplasmic domain.
      Espèces réactives Cheval Rat Souris Humain
      Species Reactivity Note Demonstrated to react with human, mouse, and rat. Predicted to react with equine and platypus based on 100% sequence homology.
      Type d'anticorps Anticorps polyclonal
      Code Entrez Gene
      Résumé Entrez Gene Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Symbole du gène
      • GluR-1
      • GRIA1
      • GluR-K1
      • GluR-A
      • GLUH1
      • GLUR1
      • GLURA
      • HBGR1
      Méthode de purification Affinity Purfied
      Code UniProt
      Résumé UniProt FUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

      >SIZE: 906 amino acids; 101506 Da

      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2 and RASGRF2 (By similarity).

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Interaction with CACNG2 promotes cell surface expression (By similarity).

      TISSUE SPECIFICITY: Widely expressed in brain.

      PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).

      SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.

      MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
      Informations physico-chimiques
      Dimensions
      Informations sur les matériaux
      Informations toxicologiques
      Informations de sécurité selon le SGH
      Informations sur la sécurité
      Notifications sur l'utilisation du produit
      Assurance Qualité Evaluated by Western Blot in mouse brain lysate.

      Western Blot Analysis: 1:1000 dilution of this antibody detected Glutamate receptor 1 (GluR1) on 10 µg of mouse brain lysate.
      Notification sur l'utilisation
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Informations relatives au stockage et à l'expédition
      Conditions de stockage Stable for 1 year at 2-8°C from date of receipt.
      Informations sur l'emballage
      Taille du matériau 200 µL
      Informations sur le transport
      Information complémentaire
      Caractéristiques

      Documentation

      Certificats d'analyse

      TitreNuméro de lot
      Anti-GluR1 - LV1446043A LV1446043A
      Anti-GluR1 - LV1519215 LV1519215
      Anti-GluR1 - LV1563305 LV1563305
      Anti-GluR1 - LV1619032 LV1619032
      Anti-GluR1 - LV1620620 LV1620620
      Anti-GluR1 - LV1623825 LV1623825
      Anti-GluR1 - LV1726018 LV1726018
      Anti-Glutamate receptor 1 - 2121763 2121763
      Anti-Glutamate receptor 1 - 2328021 2328021
      Anti-Glutamate receptor 1 - 2428717 2428717
      Anti-Glutamate receptor 1 - 2459591 2459591
      Anti-Glutamate receptor 1 - 2019967 2019967
      Anti-Glutamate receptor 1 - 2035064 2035064
      Anti-Glutamate receptor 1 - 2193310 2193310
      Anti-Glutamate receptor 1 - 2207172 2207172
      Anti-Glutamate receptor 1 - 2294344 2294344
      Anti-Glutamate receptor 1 - 2510279 2510279
      Anti-Glutamate receptor 1 - LV1740049 LV1740049
      Anti-Glutamate receptor 1 - LV1759223 LV1759223
      Anti-Glutamate receptor 1 - LV1773598 LV1773598
      Anti-Glutamate receptor 1 - NG1841053 NG1841053
      Anti-Glutamate receptor 1 - NG1849977 NG1849977
      Anti-Glutamate receptor 1 - NG1902000 NG1902000
      Anti-Glutamate receptor 1 -2484242 2484242
      Anti-Glutamate receptor 1 -2554887 2554887

      Références bibliographiques | 51 Disponible | Voir toutes les références

      Aperçu de la référence bibliographiqueApplicationEspèceNº PubMed
      Select 3',5'-cyclic nucleotide phosphodiesterases exhibit altered expression in the aged rodent brain.
      Kelly, Michy P, et al.
      Cell. Signal., 26: 383-97 (2014) 2014

      Afficher le résumé
      24184653
      Postsynaptic density scaffold SAP102 regulates cortical synapse development through EphB and PAK signaling pathway.
      Murata, Yasunobu and Constantine-Paton, Martha
      J. Neurosci., 33: 5040-52 (2013) 2013

      Afficher le résumé
      23486974
      TNF-α Downregulates Inhibitory Neurotransmission through Protein Phosphatase 1-Dependent Trafficking of GABAA Receptors.
      Pribiag, Horia and Stellwagen, David
      J. Neurosci., 33: 15879-93 (2013) 2013

      Afficher le résumé
      24089494
      Selective loss of AMPA receptors at corticothalamic synapses in the epileptic stargazer mouse.
      Z Barad,O Shevtsova,G W Arbuthnott,B Leitch
      Neuroscience 217 2012

      Afficher le résumé
      22609941
      Genetic Deletion of NR3A Accelerates Glutamatergic Synapse Maturation.
      Maile A Henson,Rylan S Larsen,Shelikha N Lawson,Isabel Pérez-Otaño,Nobuki Nakanishi,Stuart A Lipton,Benjamin D Philpot
      PloS one 7 2012

      Afficher le résumé
      22870318
      Altered glutamate receptor function in the cerebellum of the Ppt1-/- mouse, a murine model of infantile neuronal ceroid lipofuscinosis.
      Rozzy Finn,Attila D Kovács,David A Pearce
      Journal of neuroscience research 90 2012

      Afficher le résumé
      21971706
      Deficits in LTP Induction by 5-HT2A Receptor Antagonist in a Mouse Model for Fragile X Syndrome.
      Zhao-Hui Xu,Qi Yang,Lan Ma,Shui-Bing Liu,Guang-Sheng Chen,Yu-Mei Wu,Xiao-Qiang Li,Gang Liu,Ming-Gao Zhao
      PloS one 7 2012

      Afficher le résumé
      23119095
      Rapid enrichment of presynaptic protein in boutons undergoing classical conditioning is mediated by brain-derived neurotrophic factor.
      Li, W and Keifer, J
      Neuroscience, 203: 50-8 (2012) 2012

      Afficher le résumé
      22202461
      Distinct regional and subcellular localization of the actin-binding protein filamin a in the mature rat brain.
      Yoav Noam,Lise Phan,Shawn McClelland,Erik M Manders,Markus U Ehrengruber,Wytse J Wadman,Tallie Z Baram,Yuncai Chen
      The Journal of comparative neurology 520 2012

      Afficher le résumé
      22434607
      Two-stage AMPA receptor trafficking in classical conditioning and selective role for glutamate receptor subunit 4 (tGluA4) flop splice variant.
      Zheng, Zhaoqing, et al.
      J. Neurophysiol., 108: 101-11 (2012) 2012

      Afficher le résumé
      22490558